Quintero J C, Wesley R D, Whyard T C, Gregg D, Mebus C A
Am J Vet Res. 1986 May;47(5):1125-31.
The association of African swine fever virus (ASFV) with swine erythrocytes in vivo, in high titers, was verified by inoculating 30 pigs with 17 ASFV isolates and assaying their plasma and washed erythrocyte fractions for residual virus. Viral antigens were specifically localized on the surface of in vitro and in vivo swine erythrocytes, using the fluorescent antibody technique and 3 monoclonal antibodies specific for ASFV. The same monoclonal antibodies immunoprecipitated virus-specific polypeptides of molecular weights 13 kd and 73 kd from ASFV-infected Vero cells. Erythrocytes from viremic swine infected with Lisbon-60, Dominican Republic, Badajoz-M98, or Cameroon isolates of ASFV were studied by transmission electron microscopy. Virus was found in membrane depressions at the surface of erythrocytes. These surface depressions resembled stages of smooth surfaced pits. Erythrocytes from viremic pigs were fragile osmotically.
通过用17株非洲猪瘟病毒（ASFV）分离株接种30头猪，并检测其血浆和洗涤后的红细胞组分中的残留病毒，验证了ASFV在体内与猪红细胞的高滴度关联。使用荧光抗体技术和3种针对ASFV的单克隆抗体，病毒抗原被特异性定位在体外和体内猪红细胞的表面。相同的单克隆抗体从感染ASFV的Vero细胞中免疫沉淀出分子量为13kd和73kd的病毒特异性多肽。通过透射电子显微镜研究了感染了ASFV里斯本-60、多米尼加共和国、巴达霍斯-M98或喀麦隆分离株的病毒血症猪的红细胞。在红细胞表面的膜凹陷处发现了病毒。这些表面凹陷类似于光滑表面小窝的阶段。病毒血症猪的红细胞在渗透压方面很脆弱。
