蛋白质冠层对黑色素瘤癌细胞摄取聚乙二醇化玉米醇溶蛋白胶束的影响有限。

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核心技术专利：CN118964589B侵权必究

Suppr 超能文献

核心技术专利：CN118964589B侵权必究

Meewan Jitkasem, Somani Sukrut, Laskar Partha, Irving Craig, Mullin Margaret, Woods Stuart, Roberts Craig W, Alzahrani Abdullah R, Ferro Valerie A, McGill Suzanne, Weidt Stefan, Burchmore Richard, Dufès Christine

Strathclyde Institute of Pharmacy and Biomedical Sciences, University of Strathclyde, 161 Cathedral Street, Glasgow G4 0RE, UK.

Department of Immunology and Microbiology, University of Texas Health Rio Grande Valley, 5300 North L Street 881 Madison, McAllen, TX 78504, USA.

Pharmaceutics. 2022 Feb 18;14(2):439. doi: 10.3390/pharmaceutics14020439.

The formation of a protein layer "corona" on the nanoparticle surface upon entry into a biological environment was shown to strongly influence the interactions with cells, especially affecting the uptake of nanomedicines. In this work, we present the impact of the protein corona on the uptake of PEGylated zein micelles by cancer cells, macrophages, and dendritic cells. Zein was successfully conjugated with poly(ethylene glycol) (PEG) of varying chain lengths (5K and 10K) and assembled into micelles. Our results demonstrate that PEGylation conferred stealth effects to the zein micelles. The presence of human plasma did not impact the uptake levels of the micelles by melanoma cancer cells, regardless of the PEG chain length used. In contrast, it decreased the uptake by macrophages and dendritic cells. These results therefore make PEGylated zein micelles promising as potential drug delivery systems for cancer therapy.

纳米颗粒进入生物环境后，其表面会形成蛋白质层“冠”，这被证明会强烈影响与细胞的相互作用，尤其是影响纳米药物的摄取。在这项工作中，我们展示了蛋白质冠对癌细胞、巨噬细胞和树突状细胞摄取聚乙二醇化玉米醇溶蛋白胶束的影响。玉米醇溶蛋白成功地与不同链长（5K和10K）的聚乙二醇（PEG）共轭，并组装成胶束。我们的结果表明，聚乙二醇化赋予了玉米醇溶蛋白胶束隐身效果。无论使用何种PEG链长，人血浆的存在都不会影响黑色素瘤癌细胞对胶束的摄取水平。相比之下，它降低了巨噬细胞和树突状细胞的摄取。因此，这些结果使得聚乙二醇化玉米醇溶蛋白胶束有望成为癌症治疗的潜在药物递送系统。