Kadoglidou Kalliopi, Irakli Maria, Boutsika Anastasia, Mellidou Ifigeneia, Maninis Nikolas, Sarrou Eirini, Georgiadou Vasiliki, Tourvas Nikolaos, Krigas Nikos, Moysiadis Theodoros, Grigoriadou Katerina, Maloupa Eleni, Xanthopoulou Aliki, Ganopoulos Ioannis
Institute of Plant Breeding and Genetic Resources, ELGO-DIMITRA, Thermi, GR-57001 Thessaloniki, Greece.
Department of Computer Science, School of Sciences and Engineering, University of Nicosia, Nicosia 2417, Cyprus.
Plants (Basel). 2022 Feb 21;11(4):573. doi: 10.3390/plants11040573.
The traditionally edible aerial parts of rock samphire ( L.) could be a valuable functional food or feed ingredient due to their high antioxidant capacity, ascorbic acid content, and rich content in secondary metabolites such as phenolics and flavonoids. The first objective of this study was to evaluate eighteen genotypes derived from different regions of Greece regarding the phytochemical contents of their soluble extracts in total phenolics, total flavonoids, and individual polyphenols as determined by LC-MS analysis, as well as ascorbic acid content and their antioxidant capacity as determined by different assays, including ABTS (2,2-azino-bis-3-ethylbenzothiazoline-6-sulfonic acid), DPPH (2,2-diphenyl-1-picrylhydrazyl radical scavenging activity), and FRAP (ferric reducing antioxidant power) assays. The second objective of the study was the molecular characterization of native Greek genotypes. Great variation among genotypes was observed in terms of the antioxidant capacity, ascorbic acid content, and phenolic compounds (total phenolic content and total flavonoid content), as well as in caffeolquinic acids and flavonoids. The principal component analysis highlighted genotypes with a higher potential in antioxidants and polyphenolics. The most promising genotypes were G9 from Kefalonia, followed by G4 from Ikaria, where both clearly exhibited a similar response with high values of evaluated traits. The molecular characterization of genotypes revealed low variability and low to moderate genetic diversity between populations. Our data indicated that the rock samphire germplasm collection from the Balkan Botanic Garden of Kroussia could serve as an important source of documented genetic material and, thus, it is suggested for further investigation to provide insight regarding cultivation and agro-processing aspects, artificial selection, or plant breeding aimed at developing genotypes of high-bioactive value.
岩海蓬子（L.）传统上可食用的地上部分因其高抗氧化能力、抗坏血酸含量以及富含酚类和黄酮类等次生代谢物，可能成为一种有价值的功能性食品或饲料成分。本研究的首要目标是评估源自希腊不同地区的18个基因型，通过LC-MS分析测定其可溶性提取物中总酚、总黄酮和单个多酚的植物化学含量，以及通过包括ABTS（2,2-联氮-双-3-乙基苯并噻唑啉-6-磺酸）、DPPH（2,2-二苯基-1-苦基肼自由基清除活性）和FRAP（铁还原抗氧化能力）测定等不同分析方法测定的抗坏血酸含量及其抗氧化能力。该研究的第二个目标是对希腊本土基因型进行分子表征。在抗氧化能力、抗坏血酸含量、酚类化合物（总酚含量和总黄酮含量）以及咖啡酰奎宁酸和黄酮类方面，观察到基因型之间存在很大差异。主成分分析突出了在抗氧化剂和多酚方面具有较高潜力的基因型。最有前景的基因型是来自凯法利尼亚岛的G9，其次是来自伊卡里亚岛的G4，两者在评估性状方面均表现出相似的高值响应。基因型的分子表征显示群体间变异性低，遗传多样性低至中等。我们的数据表明，来自克鲁西亚巴尔干植物园的岩海蓬子种质资源库可作为重要的文献化遗传材料来源，因此建议进一步研究，以深入了解栽培和农业加工方面、人工选择或旨在培育具有高生物活性价值基因型的植物育种。
