Department of Public Health and Occupational Medicine, Mie University Graduate School of Medicine, 2-174 Edobashi, Tsu-shi 514-8507, Japan.
Department of Clinical Research, National Hospital Organization, Mie National Hospital, Tsu-shi 514-0125, Japan.
Viruses. 2022 Jan 18;14(2):173. doi: 10.3390/v14020173.
Few studies have shown the presence of norovirus (NoV) RNA in blood circulation but there is no data on norovirus antigenemia. We examined both antigenemia and RNAemia from the sera of children with NoV infections and studied whether norovirus antigenemia is correlated with the levels of norovirus-specific antibodies and clinical severity of gastroenteritis. Both stool and serum samples were collected from 63 children admitted to Mie National Hospital with acute NoV gastroenteritis. Norovirus antigen and RNA were detected in sera by ELISA and real-time RT-PCR, respectively. NoV antigenemia was found in 54.8% (34/62) and RNAemia in 14.3% (9/63) of sera samples. Antigenemia was more common in the younger age group (0-2 years) than in the older age groups, and most patients were male. There was no correlation between stool viral load and norovirus antigen (NoV-Ag) levels (r = -0.063; Cl -0.3150 to 0.1967; = 0.6251). Higher levels of acute norovirus-specific IgG serum antibodies resulted in a lower antigenemia OD value ( = 61; r = -0.4258; CI -0.62 to -0.19; = 0.0006). Norovirus antigenemia occurred more commonly in children under 2 years of age with NoV-associated acute gastroenteritis. The occurrence of antigenemia was not correlated with stool viral load or disease severity.
仅有少数研究显示诺如病毒（NoV）RNA 存在于血液循环中，但尚无关于诺如病毒抗原血症的数据。我们检测了诺如病毒感染患儿血清中的抗原血症和 RNA 血症，并研究了诺如病毒抗原血症是否与诺如病毒特异性抗体水平和胃肠炎临床严重程度相关。从因急性诺如病毒胃肠炎入住 Mie 国立医院的 63 名儿童中采集粪便和血清样本。通过 ELISA 和实时 RT-PCR 分别检测血清中的诺如病毒抗原和 RNA。在 63 份血清样本中，有 54.8%（34/62）检测到诺如病毒抗原血症，14.3%（9/63）检测到 RNA 血症。抗原血症在年龄较小的组（0-2 岁）中更为常见，且大多数患者为男性。粪便病毒载量与诺如病毒抗原（NoV-Ag）水平之间无相关性（r = -0.063；Cl -0.3150 至 0.1967； = 0.6251）。急性诺如病毒特异性 IgG 血清抗体水平较高，导致抗原血症 OD 值较低（ = 61；r = -0.4258；CI -0.62 至 -0.19； = 0.0006）。诺如病毒抗原血症在年龄小于 2 岁的与诺如病毒相关的急性胃肠炎患儿中更为常见。抗原血症的发生与粪便病毒载量或疾病严重程度无关。
