Nano-Convergence Manufacturing Systems Research Division, Korea Institute of Machinery and Materials (KIMM), 156 Gajeongbuk-ro, Yuseong-gu, Daejeon 304-343, Republic of Korea; School of Materials Science and Engineering, Department of Biomedical Science and Engineering, Gwangju Institute of Science and Technology, 123 Cheomdan-gwagiro, Buk-gu, Gwangju 500-712, Republic of Korea.
School of Materials Science and Engineering, Department of Biomedical Science and Engineering, Gwangju Institute of Science and Technology, 123 Cheomdan-gwagiro, Buk-gu, Gwangju 500-712, Republic of Korea.
Int J Biol Macromol. 2022 Apr 30;205:520-529. doi: 10.1016/j.ijbiomac.2022.02.012. Epub 2022 Feb 23.
Bioprinting is an emerging technology for manufacturing cell-laden three-dimensional (3D) scaffolds, which are used to fabricate complex 3D constructs and provide specific microenvironments for supporting cell growth and differentiation. The development of bioinks with appropriate printability and specific bioactivities is crucial for bioprinting and tissue engineering applications, including bone tissue regeneration. Therefore, to produce functional bioinks for osteoblast printing and bone tissue formation, we formulated various nanocomposite hydrogel-based bioinks using natural and biocompatible biomaterials (i.e., alginate, tempo-oxidized cellulose nanofibrils (TOCNF), and polydopamine nanoparticles (PDANPs)). Rheological studies and printability tests revealed that bioinks containing 1.5% alginate and 1.5% TOCNF in the presence or absence of PDANP (0.5%) are suitable for 3D printing. Furthermore, in vitro studies of 3D-printed osteoblast-laden scaffolds indicated that the 0.5% PDANP-incorporated bioink induced significant osteogenesis. Overall, the bioink consisting of alginate, TOCNF, and PDANPs exhibited excellent printability and bioactivity (i.e., osteogenesis).
生物打印是一种新兴的制造细胞负载三维（3D）支架的技术，用于制造复杂的 3D 结构，并为支持细胞生长和分化提供特定的微环境。开发具有适当可打印性和特定生物活性的生物墨水对于生物打印和组织工程应用至关重要，包括骨组织再生。因此，为了生产用于成骨细胞打印和骨组织形成的功能性生物墨水，我们使用天然和生物相容性生物材料（即藻酸盐、氧化纤维素纳米纤维（TOCNF）和聚多巴胺纳米颗粒（PDANPs））来配方各种纳米复合水凝胶基生物墨水。流变学研究和可打印性测试表明，含有 1.5%藻酸盐和 1.5% TOCNF 的生物墨水在存在或不存在 PDANP（0.5%）的情况下适合 3D 打印。此外，3D 打印的负载有成骨细胞的支架的体外研究表明，0.5% PDANP 掺入的生物墨水诱导了显著的成骨作用。总体而言，由藻酸盐、TOCNF 和 PDANPs 组成的生物墨水表现出优异的可打印性和生物活性（即成骨作用）。
