Prokocimer M, Shaklai M, Bassat H B, Wolf D, Goldfinger N, Rotter V
Blood. 1986 Jul;68(1):113-8.
Analysis of fresh human tumors have indicated that patients with B type lymphoproliferative diseases and the majority of patients with acute lymphoblastic leukemia (ALL) express elevated levels of p53 production. It is suggested that in these human malignancies, p53 may provide a novel tool for monitoring cancer activity. Conversely, p53 is not expressed in acute myeloid leukemias, myeloproliferative diseases, or myeloid leukemic cell lines. Analysis of the p53 gene structure indicated the existence of similar patterns of p53 restriction fragments in producer and nonproducer cells, which suggests that the p53 gene is not altered in the latter. However, in one case of acute promyelocytic leukemia (APL), we have observed a rearrangement in the p53 gene. Karyotype analysis has indicated that these APL cells do not contain the typical 15;17 translocation. In other APL patients who exhibit a 15;17 translocation, we found no genomic changes of the p53, suggesting that the p53 gene, which was recently mapped to the short arm of chromosome 17 in the human, is not structurally related to the typical chromosomal break point found in the long arm of chromosome 17 of APL patients.
对新鲜人类肿瘤的分析表明，患有B型淋巴增殖性疾病的患者以及大多数急性淋巴细胞白血病（ALL）患者的p53蛋白表达水平升高。有人提出，在这些人类恶性肿瘤中，p53可能为监测癌症活动提供一种新工具。相反，p53在急性髓细胞白血病、骨髓增殖性疾病或髓细胞白血病细胞系中不表达。对p53基因结构的分析表明，在产生p53的细胞和不产生p53的细胞中存在相似的p53限制性片段模式，这表明p53基因在后者中没有改变。然而，在一例急性早幼粒细胞白血病（APL）中，我们观察到p53基因发生了重排。核型分析表明，这些APL细胞不含有典型的15;17易位。在其他表现出15;17易位的APL患者中，我们未发现p53的基因组变化，这表明最近定位于人类17号染色体短臂的p53基因在结构上与APL患者17号染色体长臂上发现的典型染色体断裂点无关。
