McKnight G L, O'Hara P J, Parker M L
Cell. 1986 Jul 4;46(1):143-7. doi: 10.1016/0092-8674(86)90868-8.
A functional cDNA from Aspergillus nidulans encoding triosephosphate isomerase (TPI) was isolated by its ability to complement a tpi1 mutation in Saccharomyces cerevisiae. This cDNA was used to obtain the corresponding gene, tpiA. Alignment of the cDNA and genomic DNA nucleotide sequences indicated that tpiA contains five introns. The intron positions in the tpiA gene were compared with those in the TPI genes of human, chicken, and maize. One intron is present at an identical position in all four organisms, two other introns are located in similar positions in A. nidulans and maize, and the remaining two introns are unique to A. nidulans. These Aspergillus-specific introns are located in regions of the protein that were predicted to be interrupted by introns based on analysis of a Go plot of chicken TPI. These comparisons are discussed in relation to the evolution of introns within TPI genes.
通过其互补酿酒酵母中tpi1突变的能力，从构巢曲霉中分离出一个编码磷酸丙糖异构酶（TPI）的功能性cDNA。该cDNA被用于获得相应的基因tpiA。cDNA和基因组DNA核苷酸序列的比对表明tpiA含有5个内含子。将tpiA基因中的内含子位置与人类、鸡和玉米的TPI基因中的内含子位置进行了比较。在所有四种生物中，有一个内含子位于相同位置，另外两个内含子在构巢曲霉和玉米中的位置相似，其余两个内含子是构巢曲霉特有的。基于对鸡TPI的Go图分析，这些曲霉特异性内含子位于预测会被内含子中断的蛋白质区域。讨论了这些比较与TPI基因内含子进化的关系。
