The Key Laboratory for Biomedical Photonics of MOE at Wuhan National Laboratory for Optoelectronics - Hubei Bioinformatics & Molecular Imaging Key Laboratory, Systems Biology Theme, Department of Biomedical Engineering, College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan, 430074, China.
The Key Laboratory for Biomedical Photonics of MOE at Wuhan National Laboratory for Optoelectronics - Hubei Bioinformatics & Molecular Imaging Key Laboratory, Systems Biology Theme, Department of Biomedical Engineering, College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan, 430074, China.
Biosens Bioelectron. 2022 Jun 1;205:114100. doi: 10.1016/j.bios.2022.114100. Epub 2022 Feb 21.
The growth of bacterial resistance to antimicrobials is a serious problem attracting much attention nowadays. To prevent the misuse and abuse of antimicrobials, it is important to carry out antibiotic susceptibility testing (AST) before clinical use. However, conventional AST methods are relatively laborious and time-consuming (18-24 h). Here, we present a hand-powered vacuum-driven microfluidic (HVM) device, in which a syringe is used as the only vacuum source for rapid generating concentration gradient of antibiotics in different chambers. The HVM device can be preassembled with various amounts of antibiotics, lyophilized, and stored for ready-to-use. Bacterial samples can be loaded into the HVM device through a simple suction step. With the assistance of Alamar Blue, the AST assay and the minimum inhibitory concentration (MIC) of different antibiotics can be investigated by comparing the growth results of bacteria in different culture chambers. In addition, a parallel HVM device was proposed, in which eight AST assays can be performed simultaneously. The results of MIC of three commonly used antibiotics against E. coli K-12 in our HVM device were consistent with those obtained by traditional method while the detection time was shortened to less than 8 h. We believe that our platform is high-throughput, cost-efficient, easy to use, and suitable for POCT applications.
细菌对抗生素的耐药性不断增强是当前一个备受关注的严重问题。为了防止抗生素的误用和滥用，在临床使用之前进行抗生素药敏试验（AST）非常重要。然而，传统的 AST 方法比较繁琐和耗时（18-24 小时）。在这里，我们展示了一种手动驱动的真空微流控（HVM）装置，其中注射器是唯一的真空源，可在不同腔室内快速生成抗生素的浓度梯度。HVM 装置可以预先装入不同数量的抗生素，冻干并储存以备随时使用。通过简单的抽吸步骤即可将细菌样本加载到 HVM 装置中。在 Alamar Blue 的协助下，通过比较不同培养室内细菌的生长结果，可以进行 AST 检测和不同抗生素的最小抑菌浓度（MIC）测定。此外，还提出了一种并行 HVM 装置，可同时进行 8 项 AST 检测。我们的 HVM 装置中三种常用抗生素对大肠杆菌 K-12 的 MIC 结果与传统方法一致，而检测时间缩短至不到 8 小时。我们相信，我们的平台具有高通量、低成本、易于使用的特点，适合 POCT 应用。
