Li Shunji, Wan Chao, Wang Bangfeng, Chen Dongjuan, Zeng Wenyi, Hong Xianzhe, Li Lina, Pang Zheng, Du Wei, Feng Xiaojun, Chen Peng, Li Yiwei, Liu Bi-Feng
The Key Laboratory for Biomedical Photonics of MOE at Wuhan National Laboratory for Optoelectronics-Hubei Bioinformatics & Molecular Imaging Key Laboratory, Systems Biology Theme, Department of Biomedical Engineering, College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan 430074, China.
Department of Laboratory Medicine, Maternal and Child Health Hospital of Hubei Province, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430070, China.
Anal Chem. 2023 Apr 11;95(14):6145-6155. doi: 10.1021/acs.analchem.3c00557. Epub 2023 Mar 30.
Low-cost, rapid, and accurate acquisition of minimum inhibitory concentrations (MICs) is key to limiting the development of antimicrobial resistance (AMR). Until now, conventional antibiotic susceptibility testing (AST) methods are typically time-consuming, high-cost, and labor-intensive, making them difficult to accomplish this task. Herein, an electricity-free, portable, and robust handyfuge microfluidic chip was developed for on-site AST, termed handyfuge-AST. With simply handheld centrifugation, the bacterial-antibiotic mixtures with accurate antibiotic concentration gradients could be generated in less than 5 min. The accurate MIC values of single antibiotics (including ampicillin, kanamycin, and chloramphenicol) or their combinations against could be obtained within 5 h. To further meet the growing demands of point-of-care testing, we upgraded our handyfuge-AST with a pH-based colorimetric strategy, enabling naked eye recognition or intelligent recognition with a homemade mobile app. Through a comparative study of 60 clinical data (10 clinical samples corresponding to six commonly used antibiotics), the accurate MICs by handyfuge-AST with 100% categorical agreements were achieved compared to clinical standard methods (area under curves, AUCs = 1.00). The handyfuge-AST could be used as a low-cost, portable, and robust point-of-care device to rapidly obtain accurate MIC values, which significantly limit the progress of AMR.
低成本、快速且准确地获取最低抑菌浓度（MIC）是限制抗菌药物耐药性（AMR）发展的关键。到目前为止，传统的抗生素敏感性测试（AST）方法通常耗时、成本高且劳动强度大，这使得它们难以完成这项任务。在此，我们开发了一种用于现场AST的无电、便携式且坚固的手摇离心机微流控芯片，称为handyfuge-AST。通过简单的手持离心操作，可在不到5分钟内生成具有精确抗生素浓度梯度的细菌 - 抗生素混合物。单一抗生素（包括氨苄青霉素、卡那霉素和氯霉素）或其组合对[具体对象未提及]的准确MIC值可在5小时内获得。为了进一步满足即时检测不断增长的需求，我们采用基于pH的比色策略对手摇离心机AST进行了升级，实现了肉眼识别或通过自制移动应用程序进行智能识别。通过对60份临床数据（对应六种常用抗生素的10个临床样本）的比较研究，与临床标准方法相比，手摇离心机AST获得了100%分类一致性的准确MIC值（曲线下面积，AUC = 1.00）。手摇离心机AST可作为一种低成本、便携式且坚固的即时检测设备，用于快速获得准确的MIC值，这显著限制了AMR的进展。
