Department of Medical Microbiology, Sanko University School of Medicine, Gaziantep, Turkey.
Department of Medical Microbiology, Sanko University School of Medicine, Gaziantep, Turkey.
Public Health. 2022 Apr;205:1-5. doi: 10.1016/j.puhe.2022.01.011. Epub 2022 Jan 25.
During COVID-19 pandemic, the absence of immunity in the population left them susceptible to infection with SARS-CoV-2; healthcare workers (HCWs) being in the highest risk group. This study intends to assess and follow up the humoral immunity in HCWs vaccinated with an inactive virus vaccine (CoronaVac).
This is a prospective observational study.
A total of 1072 HCWs were investigated for the presence of immunoglobulin G antibodies to the receptor-binding domain of the S1 subunit of the spike protein of SARS-CoV-2 after vaccination. Blood samples were obtained after 28 days of the first dose, 21 days of the second dose, and 3 months after the second dose. Detection of antispike antibodies was performed by the chemiluminescent microparticle immunoassay method (SARS-CoV-2 IgG II Quant, Abbott, Ireland). The results greater than or equal to the cutoff value of 50.0 AU/mL were reported as positive.
Four weeks after the first dose of vaccine, antispike antibodies were detected in 834/1072 (77.8%) of HCWs. Seropositivity was higher among females (84.6%) than males (70.6% p < 0.001) and was found to be highest in both women and men between the ages of 18-34 years. Antispike antibodies were detected in 1008 of 1012 (99.6%) after 21 days of the second dose and in 803 of 836 (96.1%) after 3 months of the second dose.
CoronaVac was found to be highly immunogenic after two consecutive doses performed 28 days apart to HCWs; however, the immunogenicity declined significantly (p < 0.001) after 3 months following the second dose of vaccine.
在 COVID-19 大流行期间，由于人群中缺乏免疫力，他们容易感染 SARS-CoV-2；医护人员（HCWs）属于高风险群体。本研究旨在评估和随访接种灭活病毒疫苗（科兴疫苗）的 HCWs 的体液免疫。
这是一项前瞻性观察性研究。
共调查了 1072 名 HCWs，在接种疫苗后 28 天第一剂、21 天第二剂和第二剂后 3 个月，检测针对 SARS-CoV-2 刺突蛋白 S1 亚单位受体结合域的免疫球蛋白 G 抗体。使用化学发光微粒子免疫分析法（SARS-CoV-2 IgG II Quant，雅培，爱尔兰）检测抗刺突抗体。结果大于或等于 50.0 AU/mL 的截值报告为阳性。
第一剂疫苗接种后 4 周，1072 名 HCWs 中有 834/1072（77.8%）检测到抗刺突抗体。女性（84.6%）的血清阳性率高于男性（70.6%，p<0.001），18-34 岁的女性和男性中发现的血清阳性率最高。第二剂疫苗接种后 21 天，1008/1012（99.6%）检测到抗刺突抗体，第二剂疫苗接种后 3 个月，803/836（96.1%）检测到抗刺突抗体。
科兴疫苗在相隔 28 天连续接种两剂后，对 HCWs 具有高度的免疫原性；然而，在第二剂疫苗接种后 3 个月，免疫原性显著下降（p<0.001）。
