Mahoney W C, Orf J W
Clin Chem. 1985 Mar;31(3):459-62.
The immunosuppressive drug cyclosporin A, has been successfully iodinated to a specific activity of 300 Ci per gram. 125I-labeled cyclosporin and [3H]cyclosporin are nearly equivalent as tracers in a radioimmunoassay in producing standard lines (suppression by unlabeled cyclosporin) and in assigning values to clinical samples. In addition, the [125I]-labeled cyclosporin has greater than twice the sensitivity, and it is stable to long-term storage. Use of a [125I]-labeled cyclosporin tracer is more convenient, more reproducible, more precise, and easier than the tritiated-cyclosporin alternative in radioimmunoassay of this compound.
免疫抑制药物环孢菌素A已成功碘化，比活度达到每克300居里。在放射免疫分析中，125I标记的环孢菌素和[3H]环孢菌素作为示踪剂，在生成标准曲线（未标记环孢菌素的抑制作用）以及为临床样本赋值方面几乎等效。此外，[125I]标记的环孢菌素灵敏度高于前者两倍以上，且长期储存稳定。在该化合物的放射免疫分析中，使用[125I]标记的环孢菌素示踪剂比使用氚标记的环孢菌素更方便、更具可重复性、更精确且更易于操作。
