Department of Maxillofacial Surgery, University Hospital of Lübeck, Lübeck, Germany;
Department of Maxillofacial Surgery, University Hospital of Lübeck, Lübeck, Germany.
Anticancer Res. 2022 Mar;42(3):1295-1299. doi: 10.21873/anticanres.15596.
BACKGROUND/AIM: Zoledronic acid (ZA) treatment of in vitro cultured osteoblasts (OB) results in reduction in viability, proliferation and differentiation. These effects are slightly attenuated when platelets-rich fibrin and plasma (PRF and PRP) are added. However, it is still unknown whether application of PRP/PRF on ZA-treated OB in a 3D-environment would influence the viability in relation to 2D-cultivation.
Non-treated and ZA-treated OB were cultivated in 2D conditions or seeded in a 3D collagen scaffold with and without PRP/PRF. MTT test was carried out after 5 days of colonization. 4,6-diamidino-2'-phenylindole, dihydrochloride (DAPI)-staining was performed in OB grown in 3D scaffolds to ensure spatial distribution of OB.
ZA led to a significant reduction in cell viability compared to the control group. Addition of either PRF or PRP to the 3D colonized and ZA-treated OB significantly enhanced their survival and viability in relation to 2D monolayer cultivation.
The use of 3D-scaffolds has a positive effect on OB viability, and stimulation by PRF and PRP may provide a therapeutic approach to transfer these results into clinical routine for the treatment of patients with bisphosphonate related osteonecrosis of the jaw (BR-ONJ).
背景/目的：唑来膦酸（ZA）处理体外培养的成骨细胞（OB）会导致细胞活力、增殖和分化减少。当添加富含血小板的纤维蛋白和血浆（PRF 和 PRP）时，这些影响会略有减弱。然而，目前尚不清楚在 3D 环境中应用 PRP/PRF 是否会影响 ZA 处理的 OB 的活力与 2D 培养相关。
非处理和 ZA 处理的 OB 在 2D 条件下培养或在有和没有 PRP/PRF 的 3D 胶原支架中接种。在接种后 5 天进行 MTT 试验。在 3D 支架中生长的 OB 进行 4,6-二脒基-2'-苯基吲哚二盐酸盐（DAPI）染色，以确保 OB 的空间分布。
ZA 处理与对照组相比，显著降低了细胞活力。在 3D 定植和 ZA 处理的 OB 中添加 PRF 或 PRP，与 2D 单层培养相比，显著提高了它们的存活和活力。
使用 3D 支架对 OB 活力有积极影响，PRF 和 PRP 的刺激可能为将这些结果转化为治疗接受双膦酸盐相关颌骨骨坏死（BR-ONJ）患者的临床常规提供一种治疗方法。
