Koup J R, Brodsky B
Am Rev Respir Dis. 1978 Jun;117(6):1135-8. doi: 10.1164/arrd.1978.117.6.1135.
A comparison of the new homogeneous enzyme-multiplied immunoassay technique (EMIT, Syva) with a high-pressure liquid chromatographic technique for the determination of theophylline concentration is presented. The accuracy, precision, and specificity of the methods were compared. In addition, 100 samples from 61 pediatric patients receiving theophylline were assayed by both methods. The accuracy, precision, and specificity of the enzyme immunoassay support the use of this method for the quantification of theophylline concentration in clinical specimens. A significant correlation (R = 0.981, P less than 0.001) was found between results generated by enzyme immunoassay and high-pressure liquid chromatography for patient samples. The regression line relating these results had an intercept of 0.22 microgram per ml, a slope of 1.05, and a standard error of the estimate of 1.36 microgram per ml.
本文介绍了一种新型均相酶放大免疫分析技术（EMIT，Syva公司）与高压液相色谱技术在测定茶碱浓度方面的比较。对这两种方法的准确性、精密度和特异性进行了比较。此外，还采用这两种方法对61例接受茶碱治疗的儿科患者的100份样本进行了检测。酶免疫分析的准确性、精密度和特异性支持将该方法用于临床样本中茶碱浓度的定量分析。酶免疫分析和高压液相色谱法对患者样本检测结果之间存在显著相关性（R = 0.981，P < 0.001）。这些结果的回归线截距为每毫升0.22微克，斜率为1.05，估计标准误差为每毫升1.36微克。
