Department of Veterinary Pathology and Microbiology, Faculty of Veterinary Medicine, Universiti Putra Malaysia, Selangor, Malaysia.
Department of Veterinary Laboratory Diagnosis, Faculty of Veterinary Medicine, Universiti Putra Malaysia, Selangor, Malaysia.
Pak J Pharm Sci. 2022 Jan;35(1):113-121.
Edible bird's nest (EBN) is one of the natural products believed to pose health-enhancing properties. To provide a better insight into the protective role of EBN from a toxicological perspective, acetaminophen (APAP) as a common hepatotoxicant is chosen. This study focuses on the regenerative response of prophylaxis EBN extract in APAP-induced liver injury (AILI) of mice model. Eighty (80) ICR mice were assigned to groups of control, APAP (500 mg/kg), silymarin (200 mg/kg), and prophylactic EBN (60, 120 and 250mg/kg). The EBN and silymarin were orally administered daily for 7 days followed by an APAP intraperitoneal induction. Animals were sacrificed at 5, 10 and 24 hours post-APAP dosing (hpd). Liver samples were processed for hematoxylin and eosin (H&E) staining and proliferating cell nuclear antigen (PCNA) immunostaining. Significant differences in histological changes between APAP and prophylactic EBN groups were observed at 10 hpd with complete liver recovery for all groups at 24 hpd except for EBN 250 that continuously showed injuries. Hepatocyte proliferation was initiated at 5 hpd in EBN 60 and 120, while at 24 hpd, EBN 120 and 250 expressed higher PCNA-stained hepatocytes. The hepatoprotective role of EBN was shown earlier in EBN 60 and 120, while cellular proliferation delay in EBN 250. In conclusion, EBN has the potential as a prophylactic liver supplement to accelerate hepatic regeneration in the AILI model.
燕窝（EBN）是一种天然产物，被认为具有增强健康的特性。为了从毒理学角度更深入地了解 EBN 的保护作用，选择了对乙酰氨基酚（APAP）作为常见的肝毒性物质。本研究聚焦于 EBN 提取物在 APAP 诱导的小鼠肝损伤（AILI）模型中的再生反应。将 80 只 ICR 小鼠分为对照组、APAP（500mg/kg）组、水飞蓟素（200mg/kg）组和预防用 EBN（60、120 和 250mg/kg）组。EBN 和水飞蓟素每天口服给药 7 天，然后腹腔内注射 APAP。在 APAP 给药后 5、10 和 24 小时（hpd）处死动物。对肝组织进行苏木精和伊红（H&E）染色和增殖细胞核抗原（PCNA）免疫染色。在 10 hpd 时，APAP 组和预防用 EBN 组之间的组织学变化有显著差异，除了 EBN 250 组持续显示损伤外，所有组在 24 hpd 时均完全恢复肝脏。EBN 60 和 120 组在 5 hpd 时开始出现肝细胞增殖，而在 24 hpd 时，EBN 120 和 250 组表达了更高的 PCNA 染色的肝细胞。EBN 60 和 120 组更早显示出 EBN 的肝保护作用，而 EBN 250 组的细胞增殖延迟。总之，EBN 有可能作为一种预防性肝脏补充剂，加速 AILI 模型中的肝再生。
