Sodnom-Ish Buyanbileg, Eo Mi Young, Myoung Hoon, Lee Jong Ho, Kim Soung Min
Department of Oral and Maxillofacial Surgery, School of Dentistry, Dental Research Institute, Seoul National University, Seoul, Korea.
J Korean Assoc Oral Maxillofac Surg. 2022 Feb 28;48(1):3-12. doi: 10.5125/jkaoms.2022.48.1.3.
Selection of potential disease-specific biomarkers from saliva or epithelial tissues through next generation sequencing (NGS)-based protein studies has recently become possible. The early diagnosis of oral squamous cell carcinoma (OSCC) has been difficult, if not impossible, until now due to the lack of an effective OSCC biomarker and efficient molecular validation method. The aim of this study was to summarize the advances in the application of NGS in cancer research and to propose potential proteomic and genomic saliva biomarkers for NGS-based study in OSCC screening and diagnosis programs. We have reviewed four categories including definitions and use of NGS, salivary biomarkers and OSCC, current biomarkers using the NGS-based technique, and potential salivary biomarker candidates in OSCC using NGS.
通过基于下一代测序（NGS）的蛋白质研究从唾液或上皮组织中筛选潜在的疾病特异性生物标志物，近来已成为可能。由于缺乏有效的口腔鳞状细胞癌（OSCC）生物标志物和高效的分子验证方法，直到现在，OSCC的早期诊断即便不是不可能，也是困难重重。本研究的目的是总结NGS在癌症研究中的应用进展，并提出基于NGS的蛋白质组学和基因组学唾液生物标志物，用于OSCC筛查和诊断项目。我们综述了四类内容，包括NGS的定义和应用、唾液生物标志物与OSCC、基于NGS技术的现有生物标志物，以及利用NGS在OSCC中筛选潜在唾液生物标志物候选物。
