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在黑豆((L.)Hepper)中,具有稳定的新 QTL 区域赋予了对 MYMV 病的抗性及其遗传。

Novel and stable QTL regions conferring resistance to MYMV disease and its inheritance in blackgram ( (L.) Hepper).

机构信息

National Pulses Research Centre, Tamil Nadu Agricultural University, Vamban 622 303, India.

出版信息

J Genet. 2022;101.

Abstract

Mungbean yellow mosaic virus (MYMV) disease is a significant constraint for blackgram production. The present study employed a mapping population derived from a cross between susceptible (MDU 1) and resistant (TU 68) genotypes to identify quantitative trait loci (QTL) associated with MYMV disease resistance in addition to bruchine resistance loci identified from the previous study. Phenotyping was carried out in F2 generation under the disease spreader row method at field condition. Disease score observations were carried out 60 days after sowing (DAS). The chi-square goodness of fit test revealed inhibitory gene action with two genes controlling the expression of resistance to MYMV disease. However, QTL analysis revealed one major QTL region, i.e. _ at LG 10 responsible for MYMV disease score at 60 DAS, accounted for 21 per cent of variation. The identified QTL has the flanking markers as CEDG180 and CEDG116. Hence, the QTL, _ may be utilized in the breeding of MYMV disease resistance. Further, the marker-assisted introgression of both the MYMV and bruchine resistance QTLs can be performed in the near future.

摘要

菜豆黄花叶病毒(MYMV)病是影响绿豆生产的重要限制因素。本研究利用易感(MDU 1)和抗性(TU 68)基因型杂交产生的作图群体,除了从先前研究中鉴定出的抗虫肽抗性基因座外,还鉴定了与 MYMV 抗病性相关的数量性状基因座(QTL)。在田间条件下采用病情扩展株系法对 F2 代进行表型分析。播种后 60 天(DAS)进行病情评分观察。卡方拟合优度检验显示抑制基因作用,有两个基因控制对 MYMV 病的抗性表达。然而,QTL 分析显示一个主要的 QTL 区域,即 LG10 上的 _,负责 60 DAS 时的 MYMV 病评分,占变异的 21%。鉴定的 QTL 具有侧翼标记 CEDG180 和 CEDG116。因此, _ 可用于 MYMV 抗病性的选育。此外,在不久的将来可以进行 MYMV 和抗虫肽抗性 QTL 的标记辅助导入。

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