Xiang Hai, Fang Yi, Tan Zhiliang, Zhong Rongzhen
Jilin Provincial Key Laboratory of Grassland Farming, Northeast Institute of Geography and Agroecology, Chinese Academy of Sciences, Changchun, China.
College of Advanced Agricultural Science, University of Chinese Academy of Sciences, Beijing, China.
Front Microbiol. 2022 Feb 11;12:797746. doi: 10.3389/fmicb.2021.797746. eCollection 2021.
The objective of this study was to investigate associations between gastrointestinal microbiota and protein metabolism of lambs infected with . Sixteen male Ujumqin lambs (initial body weight = 32.4 ± 3.9 kg) were dewormed and randomly assigned to 2 equal groups, to be infected or not infected with (GIN and CON, respectively). The experiment lasted 77 days. The GIN lambs had lower packed cell volume (PCV) and increased wormegg count (WEC) after 14 days. Furthermore, in infected lambs, there were decreases in apparent digestibility of dry matter ( = 0.011), crude protein ( = 0.004) and ether extract ( = 0.007), as well as decreased ruminal pepsin ( < 0.001) and lipase ( = 0.032) activity but increased ruminal α-amylase ( = 0.004) and cellulase activity ( = 0.002), and decreased jejunal α-amylase activity ( = 0.033). In addition, infection with decreased alpha diversity of the gastrointestinal microbial community in the rumen, abomasum and duodenum, although microbiota associated with carbohydrate and proteolytic metabolism were increased and up to 32 KEGG pathways in the duodenum were predicted to be significantly affected. In conclusion, infection in lambs altered the gastrointestinal microbial community composition and disturbed protein digestion and allocation of absorbed amino acids. These results provided insights into consequences of infection in lambs and could facilitate development of novel nutritional strategies to improve animal health.
本研究的目的是调查感染[寄生虫名称未给出]的羔羊胃肠道微生物群与蛋白质代谢之间的关联。16只雄性乌珠穆沁羔羊（初始体重 = 32.4 ± 3.9千克）进行驱虫后随机分为2组，分别感染或不感染[寄生虫名称未给出]（分别为GIN组和CON组）。实验持续77天。14天后，GIN组羔羊的红细胞压积（PCV）较低，虫卵计数（WEC）增加。此外，感染羔羊的干物质表观消化率（P = 0.011）、粗蛋白表观消化率（P = 0.004）和乙醚提取物表观消化率（P = 0.007）均降低，瘤胃胃蛋白酶活性（P < 0.001）和脂肪酶活性（P = 0.032）降低，但瘤胃α -淀粉酶活性（P = 0.004）和纤维素酶活性（P = 0.002）增加，空肠α -淀粉酶活性降低（P = 0.033）。此外，感染[寄生虫名称未给出]降低了瘤胃、皱胃和十二指肠胃肠道微生物群落的α多样性，尽管与碳水化合物和蛋白水解代谢相关的微生物群增加，并且预计十二指肠中多达32条京都基因与基因组百科全书（KEGG）通路会受到显著影响。总之，羔羊感染[寄生虫名称未给出]改变了胃肠道微生物群落组成，扰乱了蛋白质消化和吸收氨基酸的分配。这些结果为羔羊感染[寄生虫名称未给出]的后果提供了见解，并有助于开发新的营养策略以改善动物健康。
