等电聚焦后的直接免疫固定。一种鉴定脑脊液和血清蛋白的改进方法。

Direct immunofixation after isoelectric focusing. An improved method for identification of cerebrospinal fluid and serum proteins.

作者信息

Stibler H

出版信息

J Neurol Sci. 1979 Jul;42(2):275-81. doi: 10.1016/0022-510x(79)90059-5.

DOI:10.1016/0022-510x(79)90059-5

PMID:90130

Abstract

An improved method for identification of CSF and serum proteins is described, using analytical isoelectric focusing followed by direct immunofixation in polyacrylamide gel. This method offers high sensitivity together with retained resolution after isoelectric focusing and is technically easy to perform. The gamma-trace protein, normal transferrin, haptoglobin and alpha 2-macroglobulin and two genetic alterations of transferrin were analyzed. The qualitative differences, previously observed on crossed immunoelectrofocusing, between transferrin and alpha 2-macroglobulin in the CSF and serum were confirmed by immunofixation. Protein components, differing in isoelectric pH by at least 0.03 pH unit and 0.5 mm apart were readily identified by this technique.

摘要

描述了一种改进的脑脊液（CSF）和血清蛋白鉴定方法，该方法采用分析性等电聚焦，随后在聚丙烯酰胺凝胶中进行直接免疫固定。此方法灵敏度高，等电聚焦后仍能保持分辨率，且技术操作简便。分析了γ-痕量蛋白、正常转铁蛋白、触珠蛋白和α2-巨球蛋白以及转铁蛋白的两种基因改变。通过免疫固定证实了先前在交叉免疫电聚焦中观察到的脑脊液和血清中转铁蛋白与α2-巨球蛋白之间的定性差异。该技术能够轻松鉴定等电pH值相差至少0.03个pH单位且相距0.5毫米的蛋白质成分。

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Direct immunofixation after isoelectric focusing. An improved method for identification of cerebrospinal fluid and serum proteins.等电聚焦后的直接免疫固定。一种鉴定脑脊液和血清蛋白的改进方法。

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