National Institute of Health Islamabad Pakistan 44000.
DBS&H CEME National University of Sciences and Technology, Islamabad, Pakistan.
Afr Health Sci. 2021 Sep;21(3):1117-1123. doi: 10.4314/ahs.v21i3.20.
The interpretation & correlation of the different laboratory parameters in positive dengue cases in order to evaluate that which laboratory test is more significant for diagnosis of Dengue.
Prospective examination of samples (patients' serum) for dengue virus of different genotype by using multiplex anti-dengue IgM, IgG. We have done NS-1 test by (ICT) immunochromatographic devices, and complete blood picture (CBC) by Sysmex XP-100.
Detection of Viral RNA in 100 patients showed effects in the total of 73 (73.0%) samples. This graphical comparison shows the whole positive cases including dengue NS-I antigen, dengue serology (IgM & IgG), total 62 positive cases of NS-I are detected, 10 positive cases of dengue IgM and 9 positive cases of IgG detected, in which Complete Blood Test (CBC) shows remarkable reduction in Platelets (32 cases) and Leucopenia in (24 positive cases).
In this research, it is concluded that the diagnosis of dengue cases is preliminary limited to initial stages i.e. CBC or sometimes dengue NS-I, as dengue IgM severity is more effective than that of Dengue NS-I & IgG. Many patients who had negative results in CBC and NS-1 testing, became positive when IgM and IgG serology testing has been done.
解释和关联登革热阳性病例的不同实验室参数，以评估哪种实验室检测对登革热的诊断更有意义。
通过使用多重抗登革病毒 IgM、IgG 对不同基因型的登革热病毒样本（患者血清）进行前瞻性检查。我们使用 ICT 免疫层析设备进行 NS-1 测试，并使用 Sysmex XP-100 进行全血细胞计数（CBC）。
在 100 名患者中，检测到 73 份（73.0%）样本的病毒 RNA。该图比较显示了包括登革热 NS-I 抗原、登革热血清学（IgM 和 IgG）在内的所有阳性病例，共检测到 62 份 NS-I 阳性，10 份登革热 IgM 阳性和 9 份 IgG 阳性，其中全血细胞计数（CBC）显示血小板（32 例）和白细胞减少症（24 例）显著减少。
在这项研究中，我们得出结论，登革热病例的诊断初步限于早期阶段，即 CBC 或有时是登革热 NS-I，因为登革热 IgM 的严重程度比登革热 NS-I 和 IgG 更有效。许多在 CBC 和 NS-1 检测中结果为阴性的患者，在进行 IgM 和 IgG 血清学检测后变为阳性。
