Jin Kai, Ye Xin, Li Sha, Li Bo, Zhang Caiqiao, Gao Changyou, Ye Juan
Department of Ophthalmology, the Second Affiliated Hospital of Zhejiang University, College of Medicine, Hangzhou, 310009, China.
Key Laboratory of Molecular Animal Nutrition of the Ministry of Education and Department of Veterinary Medicine, College of Animal Sciences, Zhejiang University, Hangzhou, 310058, China.
Graefes Arch Clin Exp Ophthalmol. 2016 Jan;254(1):83-9. doi: 10.1007/s00417-015-3144-6. Epub 2015 Sep 2.
The vascularization of an orbital implant is a key issue for reducing complications, such as exposure and infection.
Here, we developed a facile layer-by-layer assembly approach to modify porous hydroxyapatite (pHA) orbital implants with five collagen (COL)/heparin (HEP) multilayers.
SEM characterization showed that the average pore size of the pHA/(COL/HEP)5 scaffold was 316.8 ± 77.1 μm. After being coated with five COL/HEP multilayers, the mechanical strength was improved compared with that of the pHA scaffolds. The in vitro assay displayed that the pHA scaffolds covered with COL/HEP multilayers resulted in a larger number of human umbilical vein endothelial cells after being cultured for 14 days. The macroscopic evaluation and semi-quantitative vascular density analysis of the chicken chorioallantoic membrane assay showed that the pHA/(COL/HEP)5 scaffolds resulted in more intense angiogenesis than the pHA scaffolds.
These studies demonstrate that the biomembrane-mimicking coating of COL/HEP multilayers is a simple and effective strategy to endow combined biological performances of pHA orbital implants and to potentially reduce implant-related complications.
眼眶植入物的血管化是减少诸如暴露和感染等并发症的关键问题。
在此，我们开发了一种简便的逐层组装方法，用五层胶原蛋白（COL）/肝素（HEP）多层膜修饰多孔羟基磷灰石（pHA）眼眶植入物。
扫描电子显微镜表征显示，pHA/（COL/HEP）5支架的平均孔径为316.8±77.1μm。在用五层COL/HEP多层膜包被后，其机械强度相较于pHA支架有所提高。体外试验表明，覆盖有COL/HEP多层膜的pHA支架在培养14天后产生了更多的人脐静脉内皮细胞。鸡胚绒毛尿囊膜试验的宏观评估和半定量血管密度分析表明，pHA/（COL/HEP）5支架比pHA支架产生更强的血管生成。
这些研究表明，COL/HEP多层膜的生物膜模拟涂层是赋予pHA眼眶植入物综合生物学性能并潜在减少植入物相关并发症的一种简单有效的策略。
