Division of Oncology, Clinical Sciences in Lund, Lund University and Skåne University Hospital, Lund, Sweden.
Department of Hematology, Oncology and Radiation Physics, Skåne University Hospital, Lund, Sweden.
Oncotarget. 2022 Feb 22;13:439-453. doi: 10.18632/oncotarget.28204. eCollection 2022.
Vitamin C may impact the efficiency of radiation therapy (RT) in breast cancer. The effects of RT alone or in combination with vitamin C in SKBR3, MDA-MB-231, and MCF7 cells were compared using clonogenic assay, proliferation assay (MTT), cell cycle analysis, and Western blot. Vitamin C use was assessed in 1803 breast cancer patients 2002-2017 in relation to clinicopathological features and recurrences after RT. Vitamin C combined with RT resulted in non-significant increases in colony formation and minor differences in cell cycle arrest and expression of studied proteins, compared to RT alone. Lower vitamin C doses alone or in combination with RT, resulted in higher proliferation with MTT than higher vitamin C doses in a cell line-dependent manner. Vitamin C use was associated with lower histological grade and BMI but not recurrence risk in RT-treated patients (LogRank = 0.54). Vitamin C impacted RT efficiency differently depending on breast cancer subtype and vitamin C concentration. Lower doses of vitamin C, achievable with oral administration, might increase breast cancer cell proliferation and decrease radiosensitivity. Despite vitamin C users having less aggressive tumors than non-users, the recurrence risk in RT-treated patients was similar in vitamin C users and non-users.
维生素 C 可能会影响乳腺癌的放射治疗 (RT) 效果。通过集落形成实验、增殖实验 (MTT)、细胞周期分析和 Western blot，比较了 RT 单独或与维生素 C 联合应用于 SKBR3、MDA-MB-231 和 MCF7 细胞的效果。在 2002 年至 2017 年间，对 1803 名乳腺癌患者的维生素 C 使用情况进行了评估，以评估其与临床病理特征和 RT 后复发的关系。与单独使用 RT 相比，维生素 C 联合 RT 并未显著增加集落形成，细胞周期阻滞和研究蛋白表达的差异较小。单独使用或联合使用 RT 时，较低剂量的维生素 C 比高剂量的维生素 C 更能促进 MTT 细胞增殖，且这种作用具有细胞系依赖性。维生素 C 的使用与较低的组织学分级和 BMI 相关，但与 RT 治疗患者的复发风险无关（LogRank = 0.54）。维生素 C 对 RT 效率的影响因乳腺癌亚型和维生素 C 浓度而异。通过口服途径摄入较低剂量的维生素 C 可能会增加乳腺癌细胞增殖并降低放射敏感性。尽管维生素 C 使用者的肿瘤比非使用者侵袭性小，但在接受 RT 治疗的患者中，维生素 C 使用者和非使用者的复发风险相似。
