Kawarizadeh Amin, Nojoomi Farshad, Tabatabaei Mohammad, Hosseinzadeh Saeid, Farzaneh Mina
Department of Microbiology, Faculty of Medicine, Aja University of Medical Sciences, Tehran, Iran.
Department of Pathobiology, Faculty of Veterinary Medicine, Shiraz University, Shiraz, Iran.
Iran J Microbiol. 2019 Aug;11(4):305-312.
Human epithelial cells have been widely used to study the interaction between intestinal cells and pathogens, . In this study, the effect of probiotic bacteria and its supernatant on the growth inhibition, cytotoxicity and induction of apoptosis caused by Typhimurium and its adhesion to HT-29 cells were investigated.
supernatant was used to obtain the minimum inhibitory concentration. To evaluate the cytotoxicity and percent of apoptotic cells, and its supernatant (2, 4, 6 and 8% concentrations) with Typhimurium was added to HT-29 cells. The MTT assay was used in order to evaluate the cytotoxicity. Percent of apoptotic cells was reported using a fluorescence staining method. Additionally, the adhesion of Typhimurium to HT-29 cells was investigated. The effect of on the level of adhesion was also studied.
The most inhibitory effect was shown at the concentration of 80000 μg/ml supernatant of (54.77% ± 1.43). The simultaneous culture of Typhimurium with had the lowest amount of cytotoxicity and induced apoptosis among the all co-culture groups of Typhimurium with or its supernatant. The determined cytotoxicity and induced apoptosis were 26.06 % ± 3.79 and 17.63 % ± 2.14 respectively. In the adhesion test, it was observed that can significantly prevent adhesion of Typhimurium to HT-29 cell.
can reduce the adhesion, cytotoxicity and induction of apoptosis caused by Typhimurium in HT-29 cells
人类上皮细胞已被广泛用于研究肠道细胞与病原体之间的相互作用。在本研究中，研究了益生菌及其上清液对鼠伤寒沙门氏菌引起的生长抑制、细胞毒性和凋亡诱导的影响，以及其对HT-29细胞的黏附作用。
用上清液测定最低抑菌浓度。为评估细胞毒性和凋亡细胞百分比，将鼠伤寒沙门氏菌与益生菌及其上清液（浓度分别为2%、4%、6%和8%）添加到HT-29细胞中。采用MTT法评估细胞毒性。使用荧光染色法报告凋亡细胞百分比。此外，研究了鼠伤寒沙门氏菌对HT-29细胞的黏附情况。还研究了益生菌对黏附水平的影响。
在益生菌上清液浓度为80000μg/ml时显示出最大抑制作用（54.77%±1.43）。在鼠伤寒沙门氏菌与益生菌或其上清液的所有共培养组中，鼠伤寒沙门氏菌与益生菌同时培养时细胞毒性最低且诱导凋亡。测定的细胞毒性和诱导凋亡率分别为26.06%±3.79和17.63%±2.14。在黏附试验中，观察到益生菌可显著阻止鼠伤寒沙门氏菌对HT-29细胞的黏附。
益生菌可降低鼠伤寒沙门氏菌对HT-29细胞的黏附、细胞毒性和凋亡诱导作用
