The effect of on cytotoxicity and apoptosis induced by Typhimurium in HT-29 cell culture.

BACKGROUND AND OBJECTIVES

Human epithelial cells have been widely used to study the interaction between intestinal cells and pathogens, . In this study, the effect of probiotic bacteria and its supernatant on the growth inhibition, cytotoxicity and induction of apoptosis caused by Typhimurium and its adhesion to HT-29 cells were investigated.

MATERIALS AND METHODS

supernatant was used to obtain the minimum inhibitory concentration. To evaluate the cytotoxicity and percent of apoptotic cells, and its supernatant (2, 4, 6 and 8% concentrations) with Typhimurium was added to HT-29 cells. The MTT assay was used in order to evaluate the cytotoxicity. Percent of apoptotic cells was reported using a fluorescence staining method. Additionally, the adhesion of Typhimurium to HT-29 cells was investigated. The effect of on the level of adhesion was also studied.

RESULTS

The most inhibitory effect was shown at the concentration of 80000 μg/ml supernatant of (54.77% ± 1.43). The simultaneous culture of Typhimurium with had the lowest amount of cytotoxicity and induced apoptosis among the all co-culture groups of Typhimurium with or its supernatant. The determined cytotoxicity and induced apoptosis were 26.06 % ± 3.79 and 17.63 % ± 2.14 respectively. In the adhesion test, it was observed that can significantly prevent adhesion of Typhimurium to HT-29 cell.

CONCLUSION

can reduce the adhesion, cytotoxicity and induction of apoptosis caused by Typhimurium in HT-29 cells