白细胞介素-8基因多态性rs187238、rs19465518和rs1946519与早产儿急性肾损伤的关联
The association of rs187238, rs19465518 and rs1946519 IL-8 polymorphisms with acute kidney injury in preterm infants.
作者信息
Kadi Fiva Aprilia, Yuniati Tetty, Sribudiani Yunia, Rachmadi Dedi
机构信息
Department of Child Health, Faculty of Medicine, Universitas Padjadjaran, Dr Hasan Sadikin General Hospital, Bandung, Jalan Pasteur no. 38 Bandung 40161, West Java, Indonesia.
Department Biomedical Science, Biotechnology, Molecular Biology and Genetic Faculty of Medicine, Universitas Padjadjaran, Bandung, Jalan Eiykman no. 38 Bandung 40161, West Java, Indonesia.
出版信息
Biomedicine (Taipei). 2021 Dec 1;11(4):43-50. doi: 10.37796/2211-8039.1231. eCollection 2021.
BACKGROUND
Interleukin 18 (IL-18) promoter polymorphisms (-656G > T, -607C > A, and -137G > C) affect serum IL- 18 (sIL-18) levels and are associated with renal injury.
PURPOSE
This study aimed to determine the diagnostic utility of sIL-18 and urine IL-18 (uIL-18) as biomarkers for acute kidney injury (AKI) and analyse the association of polymorphisms to AKI in preterm infants.
METHODS
Blood and urine samples were collected from 56 preterm infants with AKI and 56 without AKI to measure serum creatinine (SCr), sIL-18, and uIL-18. Genotyping of polymorphisms was performed and analysed, with AUC-ROCs analysis used to evaluate the diagnostic utility of s-/uIL-18 levels.
RESULTS
The median sIL-18 and uIL-18 levels were significantly higher than those without AKI. For a cutoff of >132 pg/mL, the sIL-18 expression had sensitivity and specificity of 80.36% and 60.71%, respectively, while for uIL-18, a cutoff of >900.7 pg/mL had sensitivity and specificity of 51.79% and 78.57%, respectively. The odds ratio of sIL-18 and uIL-18 to predict AKI in preterm infants was 5.89 (95%CI:2.31-15.02) and 4.15 (95%CI:1.58-10.89), respectively. The polymorphisms -137G > C and -656G > T were significantly associated with sIL-18 expression.
CONCLUSION
Serum and urine IL-18 levels are risk factors for and a moderate predictor of AKI in preterm infants.
背景
白细胞介素18（IL-18）启动子多态性（-656G>T、-607C>A和-137G>C）影响血清IL-18（sIL-18）水平，并与肾损伤相关。
目的
本研究旨在确定sIL-18和尿IL-18（uIL-18）作为急性肾损伤（AKI）生物标志物的诊断效用，并分析多态性与早产儿AKI的相关性。
方法
收集56例患AKI的早产儿和56例未患AKI的早产儿的血液和尿液样本，以测量血清肌酐（SCr）、sIL-18和uIL-18。进行多态性基因分型并分析，采用AUC-ROC分析评估s-/uIL-18水平的诊断效用。
结果
sIL-18和uIL-18的中位数水平显著高于未患AKI的婴儿。对于>132 pg/mL的临界值，sIL-18表达的敏感性和特异性分别为80.36%和60.71%，而对于uIL-18，>900.7 pg/mL的临界值的敏感性和特异性分别为51.79%和78.57%。sIL-18和uIL-18预测早产儿AKI的比值比分别为5.89（95%CI：2.31-15.02）和4.15（95%CI：1.58-10.89）。-137G>C和-656G>T多态性与sIL-18表达显著相关。
结论
血清和尿液IL-18水平是早产儿AKI的危险因素和中度预测指标。
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