Munir Muhammad Abdurrahman, Rahmawati Fitria, Jamal Jamia Azdina, Ibrahim Sofian, Said Mazlina Mohd, Ahmad Mohamad Syahrizal
Department of Pharmacy, Faculty of Health Sciences, Alma Ata University, 55184, Bantul, Yogyakarta, Indonesia.
Research Group of Solid State Chemistry and Catalysis, Chemistry Department, Faculty of Mathematics and Natural Sciences, Sebelas Maret University, 57126 Surakarta, Indonesia.
ACS Omega. 2023 Mar 29;8(14):13352-13361. doi: 10.1021/acsomega.3c00768. eCollection 2023 Apr 11.
Numerous analytical approaches have been developed to determine histamine levels in food samples due to its health consequences. Consuming histamine over the Food and Drug Administration (FDA)-regulated 50 mg kg limit would result in chronic toxicity. Consequently, the present study discusses a novel electrochemical approach to evaluate histamine levels in fish products via a molecularly imprinted polymer (MIP) on an electrode surface. The film was produced with electropolymerized polyurethane (PU), which maintained the histamine compound. Fourier-transform infrared (FTIR) spectroscopy was applied to verify the MIP manufactured in this study. The capability of the polymer was measured by assessing its electron shifts with cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS). Differential pulse voltammetry (DPV) was also employed to validate the sensing method. The MIP/screen-printed electrode (SPE) and non-imprinted polymer (NIP)/SPE recorded a linear response ranging from 1 to 1000 nmol L at the 1.765 and 709 nmol L detection limits. The sensing technique was subsequently utilized to determine the histamine levels in selected samples at room temperature (25 °C). Generally, the sensor allowed the accurate and precise detection of histamine in the fish samples. Furthermore, the approach could be categorized as a simple technique that is low-cost and suitable for on-site detections.
由于组胺对健康的影响，人们已经开发出多种分析方法来测定食品样品中的组胺水平。食用超过美国食品药品监督管理局（FDA）规定的50毫克/千克限量的组胺会导致慢性毒性。因此，本研究讨论了一种新颖的电化学方法，通过电极表面的分子印迹聚合物（MIP）来评估鱼制品中的组胺水平。该薄膜由电聚合聚氨酯（PU）制成，它能保留组胺化合物。采用傅里叶变换红外（FTIR）光谱法来验证本研究中制备的MIP。通过循环伏安法（CV）和电化学阻抗谱（EIS）评估聚合物的电子转移来测量其性能。差分脉冲伏安法（DPV）也用于验证传感方法。MIP/丝网印刷电极（SPE）和非印迹聚合物（NIP）/SPE在1.765和709纳摩尔/升的检测限下记录了1至1000纳摩尔/升的线性响应。随后利用该传感技术在室温（25℃）下测定选定样品中的组胺水平。一般来说，该传感器能够准确、精确地检测鱼样品中的组胺。此外，该方法可归类为一种简单、低成本且适用于现场检测的技术。
