Guerin J F, Ali H B, Rollet J, Souchier C, Czyba J C
J Androl. 1986 May-Jun;7(3):156-62. doi: 10.1002/j.1939-4640.1986.tb00901.x.
Azoospermic semen was obtained from 39 vasectomized men and 93 patients consulting for infertility. The latter underwent bioclinical investigations including measurement of plasma FSH and testicular biopsies. Carnitine content and alpha-glucosidase activity in semen samples were measured. The activity of alpha-glucosidase was determined systematically by a semiquantitative microtechnique and was verified by a spectrophotometric assay. A positive correlation was observed between carnitine and alpha-glucosidase activity. Both parameters were severely diminished in semen from the vasectomized men and the patients suffering from a complete obstruction of the genital tract. Enzyme activity was the most discriminant parameter in terms of sensitivity and specificity. The measurement of alpha-glucosidase in semen is a simple and sensitive method for determining the origin of azoospermia when used in conjunction with assays for plasma FSH.
从39名输精管结扎男性和93名因不育前来咨询的患者中获取了无精子精液。后者接受了生物临床检查，包括测定血浆促卵泡生成素（FSH）和进行睾丸活检。测定了精液样本中的肉碱含量和α - 葡萄糖苷酶活性。α - 葡萄糖苷酶活性通过半定量微技术系统测定，并通过分光光度法进行验证。观察到肉碱与α - 葡萄糖苷酶活性之间呈正相关。在输精管结扎男性和患有生殖道完全梗阻的患者的精液中，这两个参数均严重降低。就敏感性和特异性而言，酶活性是最具鉴别力的参数。当与血浆FSH检测结合使用时，精液中α - 葡萄糖苷酶的测定是确定无精子症病因的一种简单而敏感的方法。
