Sandoval L, Diaz M, Rivas F
División de Genética, Centro de Investigación Biomédica de Occidente, Guadalajara, Jalisco, México.
Arch Androl. 1995 Sep-Oct;35(2):155-8. doi: 10.3109/01485019508987867.
Among azoospermics, the activity of seminal alpha-glucosidase (alpha G) is low in obstruction (OBS) cases, but there is no agreement regarding its value in others. With the hypothesis that a more marked decrease in alpha G activity occurs in OBS than in spermatogenesis arrest (SA), the enzyme was measured in the following groups: (1) vasectomized males (n = 15), (2) azoospermics with OBS (n = 7), (3) azoospermics with SA (n = 11), and (4) fertile males (n = 15). Patients within groups 2 and 3 had a testicular volume > or = 15 mL, no clinical evidence of abnormal epididymis, normal serum gonadotropins, and histological diagnosis. alpha G activities (mU/g protein, means +/- SD) by group were (1) 131 +/- 60, (2) 312 +/- 186, (3) 728 +/- 303, and (4) 1176 +/- 374. All between-group differences were significant (t test, p < .01), except for groups 1 vs. 2 comparison (.05 > p > .01). These results support the notion that SA is associated with a decrease in alpha G that is less marked that seen in OBS. The search for germinal epithelium cells in semen also allows discrimination between these two azoospermic groups.
在无精子症患者中，梗阻性（OBS）病例的精浆α-葡萄糖苷酶（αG）活性较低，但对于其在其他病例中的价值尚无定论。基于OBS患者αG活性下降比生精阻滞（SA）患者更明显的假设，对以下几组患者的该酶进行了测定：（1）输精管结扎男性（n = 15），（2）梗阻性无精子症患者（n = 7），（3）生精阻滞性无精子症患者（n = 11），以及（4）有生育能力的男性（n = 15）。第2组和第3组患者的睾丸体积≥15 mL，无附睾异常的临床证据，血清促性腺激素正常，且经组织学诊断。各组的αG活性（mU/g蛋白，均值±标准差）分别为：（1）131±60，（2）312±186，（3）728±303，（4）1176±374。除第1组与第2组比较外（0.05＞p＞0.01），所有组间差异均具有统计学意义（t检验，p＜0.01）。这些结果支持以下观点：SA患者αG活性的下降程度不如OBS患者明显。对精液中生发上皮细胞的检测也有助于区分这两种无精子症类型。
