Guerin J F, Rollet J, Perrin P, Menezo Y, Orgiazzi A, Czyba J C
Fertil Steril. 1981 Sep;36(3):368-72. doi: 10.1016/s0015-0282(16)45740-7.
Semi-quantitative estimations of the activities of 65 enzymes were studied for cases of azoospermia in an attempt to correlate the seminal enzyme profile with the origin of the azoospermia. One single enzyme--alpha-glucosidase--showed variation. All other specificities tested gave identical results for all the azoospermic samples and for normal controls. The alpha-glucosidase activity that is present in normal semen was absent from samples from patients with azoospermia associated with a complete obstruction of the genital tract, and also from all semen samples from vasectomized subjects. This enzyme appears to be secreted by the epididymis, and the activity present in azoospermic semen can provide information concerning the origin of the azoospermia and the functional state of the epididymis. Positive but reduced alpha-glucosidase activity appears to be frequently associated with inflammatory disease. The consequences of these findings are discussed.
为了将精液酶谱与无精子症的病因相关联，对65种酶活性进行了半定量评估，研究了无精子症病例。仅有一种酶——α-葡萄糖苷酶——表现出差异。所有其他测试的特异性在所有无精子症样本和正常对照中都给出了相同的结果。正常精液中存在的α-葡萄糖苷酶活性在患有与生殖道完全梗阻相关的无精子症患者的样本中以及所有输精管切除受试者的精液样本中均不存在。这种酶似乎由附睾分泌，无精子症精液中存在的活性可以提供有关无精子症病因和附睾功能状态的信息。α-葡萄糖苷酶活性呈阳性但降低似乎经常与炎症性疾病相关。讨论了这些发现的后果。
