大肠杆菌N-乙酰神经氨酸裂解酶的完整核苷酸序列。
Complete nucleotide sequence of the E. coli N-acetylneuraminate lyase.
作者信息
Ohta Y, Watanabe K, Kimura A
出版信息
Nucleic Acids Res. 1985 Dec 20;13(24):8843-52. doi: 10.1093/nar/13.24.8843.
Abstract
The nucleotide sequence of the cloned DNA, 1,243 bp in length coding for N-acetylneuraminate lyase (N-acetylneuraminate pyruvate lyase; NPL) of Escherichia coli has been determined. Nucleotide sequence and amino acid analysis have assigned the open reading frame for NPL, starting with the ATG near its 5'terminus. The molecular weight calculated from the predicted amino acid sequence was 32,640 daltons, being in good agreement with that of a NPL subunit estimated by the SDS-PAGE method and amino acid composition. Several signal sequences conserved in the promoter regions of E. coli were found in the npl gene. They were the Shine-Dalgarno sequence, the Pribnow box and the sequence coserved in the "-35 region" and they were separated to each other with preferable spacing for an efficient transcription. Downstream from the termination codon, the inverted repeat sequence was present, followed by 4 successive T's.
摘要
已测定了克隆的大肠杆菌N - 乙酰神经氨酸裂解酶（N - 乙酰神经氨酸丙酮酸裂解酶；NPL）编码DNA的核苷酸序列，其长度为1243bp。核苷酸序列和氨基酸分析确定了NPL的开放阅读框，起始于其5'端附近的ATG。根据预测的氨基酸序列计算出的分子量为32,640道尔顿，与通过SDS - PAGE法和氨基酸组成估计的NPL亚基分子量高度一致。在npl基因中发现了大肠杆菌启动子区域中保守的几个信号序列。它们是Shine - Dalgarno序列、Pribnow框以及在“-35区域”中保守的序列，并且它们彼此之间以有利于有效转录的合适间距分开。在终止密码子下游，存在反向重复序列，其后跟着4个连续的T。
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