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嗜碱芽孢杆菌菌株170的β-内酰胺酶基因的核苷酸序列

Nucleotide sequence of the beta-lactamase gene of alkalophilic Bacillus sp. strain 170.

作者信息

Kato C, Kudo T, Watanabe K, Horikoshi K

出版信息

J Gen Microbiol. 1985 Dec;131(12):3317-24. doi: 10.1099/00221287-131-12-3317.

DOI:10.1099/00221287-131-12-3317
PMID:3938474
Abstract

A gene for the beta-lactamase from alkalophilic Bacillus sp. strain 170 was cloned in a functional state on a 1.0 kb DNA fragment and its nucleotide sequence was determined. The coding sequence showed an open reading frame of 257 amino acids, which represents the beta-lactamase precursor protein. It is considered that the signal peptide consisted of 30 amino acids including 12 hydrophobic amino acids.

摘要

嗜碱芽孢杆菌属菌株170的β-内酰胺酶基因在一个1.0 kb的DNA片段上以功能状态被克隆，并测定了其核苷酸序列。编码序列显示有一个257个氨基酸的开放阅读框，它代表β-内酰胺酶前体蛋白。据认为，信号肽由30个氨基酸组成，其中包括12个疏水氨基酸。

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