Food Chemistry and Food Development, Department of Life Technologies, University of Turku, FI-20014 Turun yliopisto, Finland.
Beijing Key Laboratory of Toxicological Research and Risk Assessment for Food Safety, Department of Toxicology, School of Public Health, Peking University, Beijing 100191, China.
Food Res Int. 2022 Mar;153:110978. doi: 10.1016/j.foodres.2022.110978. Epub 2022 Feb 7.
Anthocyanins have been shown to have prebiotic properties. This study investigated the impact of nonacylated anthocyanins and acylated anthocyanins on fecal and cecal metabolites and colonic gut microbiota in diabetic state using H nuclear magnetic resonance (NMR) metabolomics and metagenomic sequencing. Zucker diabetic fatty rats fed with high-fat diet were gavaged with nonacylated anthocyanins extracted from bilberries (NAAB) or acylated anthocyanins extracted from purple potatoes (AAPP) at daily doses of 25 and 50 mg/kg body weight for 8 weeks. Lean Zucker rats fed with normal diet (ND) and high-fat diet (Con) were used as healthy controls groups. Binned NMR spectra and sequenced gene abundance were used for data analysis. Dysbiosis of colonic microbiota and gut metabolites in the diabetic rats were observed compared to the lean Zucker rats. Both anthocyanin extracts increased cecal sugar levels and the abundance of Peptostreptococcaceae sp. and decreased the abundance of Parabacteroides spp. in colon. In addition to the increased fecal short-chain fatty acids, AAPP decreased colonic Ruminococcus torques and Lachnospiraceae bacterium 4_1_37FAA abundances and increased oxidative phosphorylation. The anthocyanin extracts modulated the gut metabolism and microbiota in diabetes, with AAPP showing more regulatory and beneficial effects on diabetes.
花色苷具有益生元特性。本研究采用氢核磁共振（NMR）代谢组学和宏基因组测序技术，研究了非酰化花色苷和酰化花色苷对糖尿病状态下粪便和盲肠代谢物以及结肠肠道微生物群的影响。给予高脂肪饮食的 Zucker 糖尿病肥胖大鼠每天灌胃 25 和 50mg/kg 体重的蓝莓非酰化花色苷（NAAB）或紫土豆酰化花色苷（AAPP）8 周。给予正常饮食（ND）和高脂肪饮食（Con）的 lean Zucker 大鼠作为健康对照组。对分箱 NMR 光谱和测序基因丰度进行数据分析。与 lean Zucker 大鼠相比，糖尿病大鼠的结肠微生物群和肠道代谢物出现了失调。两种花色苷提取物均增加了盲肠中的糖水平，增加了 Peptostreptococcaceae sp. 的丰度，并降低了结肠中 Parabacteroides spp. 的丰度。除了粪便短链脂肪酸增加外，AAPP 还降低了结肠中的 Ruminococcus torques 和 Lachnospiraceae bacterium 4_1_37FAA 的丰度，并增加了氧化磷酸化。花色苷提取物调节了糖尿病中的肠道代谢和微生物群，AAPP 对糖尿病的调节作用和有益作用更为明显。
