Chen Kang, Wei Xuetao, Pariyani Raghunath, Kortesniemi Maaria, Zhang Yumei, Yang Baoru
Food Chemistry and Food Development, Department of Life Technologies, University of Turku, FI-20014 Turun yliopisto, Finland.
Beijing Key Laboratory of Toxicological Research and Risk Assessment for Food Safety, Department of Toxicology, School of Public Health, Beijing University, Beijing 100191, China.
J Agric Food Chem. 2021 Apr 21;69(15):4423-4437. doi: 10.1021/acs.jafc.1c00130. Epub 2021 Apr 9.
Anthocyanins have been reported to possess antidiabetic effects. Recent studies indicate acylated anthocyanins have better stability and antioxidative activity compared to their nonacylated counterparts. This study compared the effects of nonacylated and acylated anthocyanins on hepatic gene expression and metabolic profile in diabetic rats, using full-length transcriptomics and H NMR metabolomics. Zucker diabetic fatty (ZDF) rats were fed with nonacylated anthocyanin extract from bilberries (NAAB) or acylated anthocyanin extract from purple potatoes (AAPP) at daily doses of 25 and 50 mg/kg body weight for 8 weeks. Both anthocyanin extracts restored the levels of multiple metabolites (glucose, lactate, alanine, and pyruvate) and expression of genes (, , , and ) involved in glycolysis and gluconeogenesis. AAPP decreased the hepatic glutamine level. NAAB regulated the expression of , , and , whereas AAPP modified the expression of , , , and . This study indicated different effects of AAPP and NAAB on the hepatic transcriptomic and metabolic profiles of diabetic rats.
据报道，花青素具有抗糖尿病作用。最近的研究表明，酰化花青素与其非酰化对应物相比具有更好的稳定性和抗氧化活性。本研究使用全长转录组学和核磁共振代谢组学，比较了非酰化和酰化花青素对糖尿病大鼠肝脏基因表达和代谢谱的影响。将Zucker糖尿病脂肪（ZDF）大鼠分别喂食越橘非酰化花青素提取物（NAAB）或紫薯酰化花青素提取物（AAPP），每日剂量为25和50mg/kg体重，持续8周。两种花青素提取物均恢复了参与糖酵解和糖异生的多种代谢物（葡萄糖、乳酸、丙氨酸和丙酮酸）的水平以及相关基因（、、、和）的表达。AAPP降低了肝脏谷氨酰胺水平。NAAB调节了、和的表达，而AAPP改变了、、、和的表达。本研究表明AAPP和NAAB对糖尿病大鼠肝脏转录组和代谢谱有不同影响。
