Institute of Agro-food Technology, Jilin Academy of Agricultural Sciences, 130033, Changchun, Jilin, China.
Department of International Cooperation, Jilin Academy of Agricultural Sciences, 130033, Changchun, Jilin, China.
Food Funct. 2022 Jul 4;13(13):7377-7391. doi: 10.1039/d2fo01165d.
Black rice and black bean have not yet been fully investigated as healthy foods for their therapeutic effects on type 2 diabetes mellitus (T2DM). In this study, we aimed to evaluate the antidiabetic effects of black rice, black bean husk anthocyanin extracts, and their combination on glycolipid metabolism, gut microbiota, and serum metabolites in T2DM rats. Black bean husk and black rice anthocyanin extracts were administered to T2DM rats by gavage for 4 weeks. The results showed that black rice and black bean husk anthocyanin extracts significantly improved blood glucose, insulin resistance, serum oxidative stress state, lipid metabolism and inflammatory cytokines levels in rats, and alleviated liver damage. Black rice and black bean husk anthocyanin extracts increased the abundance of short-chain fatty acid (SCFA) producing bacteria spp., spp., spp., and spp., changed the gut microbiota structure; activated AMPK, PI3K, and AKT; inhibited HMGCR, G6pase and PEPCK expression; and inhibited hepatic gluconeogenesis. Moreover, by adjusting the levels of urea, deoxycytidine, L-citrulline, pseudouridine, and other serum metabolites in T2DM rats, the arginine biosynthesis and pyrimidine metabolism pathways were downregulated. The above results indicated that black rice and black bean husk anthocyanin extracts had a significant impact on the development of T2DM.
黑米和黑豆在作为治疗 2 型糖尿病（T2DM）的健康食品方面的治疗作用尚未得到充分研究。在这项研究中，我们旨在评估黑米、黑豆皮花色苷提取物及其组合对 T2DM 大鼠糖脂代谢、肠道微生物群和血清代谢物的降糖作用。通过灌胃给予 T2DM 大鼠 4 周黑米粉和黑豆皮花色苷提取物。结果表明，黑米和黑豆皮花色苷提取物可显著改善大鼠的血糖、胰岛素抵抗、血清氧化应激状态、脂代谢和炎症细胞因子水平，并减轻肝损伤。黑米和黑豆皮花色苷提取物增加了短链脂肪酸（SCFA）产生菌 spp.、 spp.、 spp.和 spp.的丰度，改变了肠道微生物群结构；激活 AMPK、PI3K 和 AKT；抑制 HMGCR、G6pase 和 PEPCK 的表达；抑制肝糖异生。此外，通过调节 T2DM 大鼠血清中尿素、脱氧胞苷、L-瓜氨酸、假尿嘧啶等代谢物的水平，下调了精氨酸生物合成和嘧啶代谢途径。上述结果表明，黑米和黑豆皮花色苷提取物对 T2DM 的发生发展有显著影响。
