Department of Biotechnology, Haldia Institute of Technology, HIT Campus, Haldia, PurbaMedinipur, West Bengal, 721657, India.
Department of Biotechnology, Haldia Institute of Technology, HIT Campus, Haldia, PurbaMedinipur, West Bengal, 721657, India.
Chemosphere. 2022 Jun;297:134112. doi: 10.1016/j.chemosphere.2022.134112. Epub 2022 Feb 25.
There has been a growing interest in the scientific world in the production of biochar from natural organic wastes as potential sustainable precursors for bioremediation. Potato peel biochar was produced by slow pyrolysis method under oxygen-limited conditions and used as bio adsorbent in bioremediation of commercial pesticide having Chlorpyrifos as an active component. Chlorpyrifos is an organophosphate pesticide, highly neurotoxic, and primarily targets the central nervous system of pests and insects. The excess residues of chlorpyrifos are hazardous to environmental flora and fauna. Chlorpyrifos was treated against biochar at varying physical parameters and further optimized by using response surface methodology through Box-Behnken design (BBD). 72.06% of pesticide removal was observed post 24 h of treatment against a pesticide concentration of 1346.85 μg/ml with a biochar concentration of 1.04 mg/ml under room temperature at pH 5.04. Biochar was characterized by proximate and ultimate analysis, FTIR, and SEM-EDX. Characterization by SEM-EDX showed the surface morphology and minerals on the peel and biochar. Microgram of potato peel shows pores of larger size than biochar having many cavities with different dimensions. In the plant system, growth morphology, nutritional status, polyphenols, total antioxidant content, and free radical scavenging activity were assessed. Enhancement in presence of biochar was recorded in growth morphology and plant biomolecules including photosynthetic pigments. Better translocation of the nutrient is recorded in biochar treated plants, as evidenced by the low amount of carbohydrate and protein in treated leaves. Biocompatibility assessment of chlorpyriphos in fish erythrocytes showed 43.26% hemolysis by pesticide-treated biochar. The practical use of this approach can also be best utilized if applied to those geographical regions where the soil pH is acidic. Biochar is a marketable bio-product, which can have a positive impact in agriculture, industries, and the energy sector creating a bio-based economy with reduced environmental pollution.
科学界越来越关注利用天然有机废物生产生物炭作为生物修复的潜在可持续前体。采用限氧慢速热解方法制备马铃薯皮生物炭，并将其用作含有氯吡硫磷作为活性成分的商业农药的生物吸附剂进行生物修复。氯吡硫磷是一种有机磷农药，具有高度神经毒性，主要针对害虫和昆虫的中枢神经系统。氯吡硫磷的过量残留对环境中的动植物具有危害性。采用响应面法通过 Box-Behnken 设计（BBD）对氯吡硫磷在不同物理参数下进行处理，并进一步进行优化。在室温、pH 值为 5.04 的条件下，用 1.04mg/ml 的生物炭处理 1346.85μg/ml 的农药浓度，24 小时后可去除 72.06%的农药。采用工业分析、元素分析、傅里叶变换红外光谱和扫描电子显微镜-能谱分析（SEM-EDX）对生物炭进行了表征。SEM-EDX 分析表明，马铃薯皮和生物炭表面形貌和矿物。马铃薯皮的微孔比生物炭大，有许多不同尺寸的空腔。在植物系统中，评估了生物炭存在时的生长形态、营养状况、多酚、总抗氧化含量和自由基清除活性。在生长形态和包括光合色素在内的植物生物分子方面，均记录到生物炭的增强作用。在生物炭处理过的植物中，观察到营养物质更好的转移，这从处理过的叶片中碳水化合物和蛋白质的含量较低得到证明。对鱼类红细胞中氯吡硫磷的生物相容性评估表明，用农药处理的生物炭导致 43.26%的溶血。如果将这种方法应用于土壤 pH 值呈酸性的地理区域，那么这种方法的实际应用也可以得到最佳利用。生物炭是一种有市场价值的生物产品，可以在农业、工业和能源部门产生积极影响，创造一个减少环境污染的基于生物的经济。
