Lanzhou University Second Hospital, Lanzhou 730000, China.
Department of the orthopaedic centre, The 940th Hospital of Logistics Support Force of PLA, Lanzhou 730000, China.
Colloids Surf B Biointerfaces. 2022 May;213:112424. doi: 10.1016/j.colsurfb.2022.112424. Epub 2022 Feb 23.
Antibiotic bone cement filling technology has been widely used in the treatment of infectious bone defects for decades. However, the current treatment requires multiple complicated procedures, which would lead to pain and financial burden for patients. Repairing bone defects and control infection at the same time is the pain spot of orthopaedic area. In this study, we develop a composite scaffold that aiming at effectively repair infectious bone defects simultaneously. Vancomycin hydrochloride(Van) /Poly(lactic-co-glycolic) acid(PLGA) microspheres prepared by double emulsion method were successfully loaded into β-tricalcium phosphate scaffold through electrostatic and physical crosslinking. Full characterization, including mechanical properties, biocompatibility, in vitro release profile and antibacterial properties of the composite scaffolds(CPSFs) were performed. The rabbit osteomyelitis model based on big hole and small hole methods was established. Pharmacodynamics study, including the local bacteriostatic and osteogenic ability were evaluated by X-ray, Micro-CT and histopathology at 4 months after surgery. These findings indicate that a reliable rabbit model of local bone defect infection successfully established by big hole approach. The CPSFs with significant histocompatibility and biocompatibility could sustained release vancomycin for extended duration. It exhibited great application potential in clinical aim at the indication of local infectious bone defects.
抗生素骨水泥填充技术在治疗感染性骨缺损方面已经得到了几十年的广泛应用。然而，目前的治疗方法需要多次复杂的手术，这会给患者带来疼痛和经济负担。同时修复骨缺损和控制感染是骨科领域的痛点。在本研究中，我们开发了一种旨在有效同时修复感染性骨缺损的复合材料支架。通过复乳法成功地将盐酸万古霉素（Van）/聚乳酸-羟基乙酸共聚物（PLGA）微球加载到β-磷酸三钙支架上，通过静电和物理交联。对复合材料支架（CPSFs）进行了全面的特性研究，包括机械性能、生物相容性、体外释放曲线和抗菌性能。采用大、小孔法建立了兔骨髓炎模型。术后 4 个月通过 X 射线、Micro-CT 和组织病理学评估了药物动力学研究，包括局部抑菌和成骨能力。这些发现表明，通过大孔方法成功建立了一种可靠的局部骨缺损感染的兔模型。具有显著组织相容性和生物相容性的 CPSFs 可以持续释放万古霉素，延长释放时间。它在临床应用方面具有很大的潜力，适用于局部感染性骨缺损的治疗。
