School of Environmental and Municipal Engineering, Xi'an University of Architecture and Technology, Xi'an, 710055, China E-mail:
Shaanxi Institute for Food and Drug Control, Xi'an, 710065, China.
Water Sci Technol. 2022 Feb;85(4):1176-1190. doi: 10.2166/wst.2022.018.
The distribution characteristics of antibiotic-resistant bacteria (ARB) and antibiotic resistance genes (ARGs) in urban recreational water from different water-supply sources might be different. In this study, water samples were collected to detect the antibiotic resistance of heterotrophic bacteria to five antibiotics, and the content, phenotype, gene type and species distribution of resistant bacteria were analyzed. The results showed that the changes of bacteria resistance rate in two lakes to five kinds of antibiotics were synchronous with time, and it would reach its maximum in autumn. The detection of ARGs and int I in 80 resistance strains showed that the detection rate of tetG, tetA and int I was high. Here, 51.25% of the bacteria were doubly resistant to AMP-CTX. The 80 isolate strains were of nine genera and 19 species, among which Bacillus cereus, Escherichia coli, Aeromonas veronii, Aeromonas caviae and Raoultella ornithinolytica were the common ARB species in two lakes. Correlation analysis showed that the water temperature was significantly correlated with the content of ARB in sulfamethoxazole (SMZ) and cefotaxime (CTX) (p < 0.05), and the total phosphorus (TP) in FQ lake was significantly correlated with the content of AMP-resistant bacteria (p < 0.05), while there were no other correlations between the changes of other water quality indexes and the content of ARB (p > 0.05).
不同水源城市娱乐性水体中抗生素耐药菌（ARB）和抗生素耐药基因（ARGs）的分布特征可能存在差异。本研究采集水样，检测异养菌对 5 种抗生素的耐药性，分析了耐药菌的含量、表型、基因类型和种属分布。结果表明，两湖细菌耐药率随时间变化呈同步变化，秋季达到最大值。对 80 株耐药菌的 ARGs 和 int I 检测显示，tetG、tetA 和 int I 的检出率较高。51.25%的细菌对 AMP-CTX 呈双重耐药。80 株分离株属于 9 个属和 19 个种，其中蜡样芽胞杆菌、大肠埃希菌、维罗纳气单胞菌、豚鼠气单胞菌和 ornithinolytica 是两湖常见的 ARB 种属。相关性分析表明，温度与磺胺甲恶唑（SMZ）和头孢噻肟（CTX）中 ARB 含量呈显著相关（p<0.05），FQ 湖总磷（TP）与 AMP 耐药菌含量呈显著相关（p<0.05），而其他水质指标变化与 ARB 含量之间无其他相关性（p>0.05）。
