Zhang Songhe, Pang Si, Wang PeiFang, Wang Chao, Han Nini, Liu Bin, Han Bing, Li Yi, Anim-Larbi Kwaku
Ministry of Education Key Laboratory of Integrated Regulation and Resource Development on Shallow Lakes, College of Environment, Hohai University, Nanjing, 210098, China.
Institute of Pearl River Water Resources Protection, Pearl River Water Resources Commission of Ministry of Water Resource, Guangzhou, 510635, China.
Environ Sci Pollut Res Int. 2016 May;23(10):9984-92. doi: 10.1007/s11356-016-6237-9. Epub 2016 Feb 11.
Stormwater runoff is generally characterized as non-point source pollution. In the present study, antibiotic concentration and antibiotic susceptibilities of cultivable heterotrophic bacteria were investigated in two small shallow urban lakes before and after strong storm event. Several antibiotics, lactose-fermenting bacteria and cultivable heterotrophic bacteria concentrations increased in surface water and/or surface sediment of two small urban lakes (Lake Xuanwu and Wulongtan) after strong storm event. In general, the frequencies of bacteria showing resistance to nine antibiotics increased after storm event. Based on the 16S rRNA genes of 50 randomly selected isolates from each water sample of two lakes, Aeromonas and Bacillus were dominant genera in samples from two lakes, while genera Proteus and Lysinibacillus were the third abundant genera in Lake Xuanwu and Wulongtu, respectively. Presences of nine antibiotic resistance genes (ARGs) in the 100 isolates were detected and most of these isolates harbored at least two ARGs with different functions. The detection frequency of ARGs in Gram-negative isolates was higher than that in Gram-positive isolates. The most prevalent integron in 100 isolates was int(II) (n = 28), followed by int(I) (n = 17) and int(III) (n = 17). Our results indicate that strong storm events potentially contribute to the transfer of ARGs and antibiotic-resistant bacteria from land-sewer system to the urban Lakes.
雨水径流通常被视为非点源污染。在本研究中，对两场强降雨事件前后两个小型城市浅水湖泊中可培养异养细菌的抗生素浓度及抗生素敏感性进行了调查。两场强降雨事件后，两个小型城市湖泊（玄武湖和乌龙潭）的地表水和/或表层沉积物中的几种抗生素、乳糖发酵菌及可培养异养细菌浓度均有所增加。总体而言，强降雨事件后对九种抗生素呈现耐药性的细菌频率有所增加。基于从两个湖泊的每个水样中随机选取的50个分离株的16S rRNA基因，气单胞菌属和芽孢杆菌属是两个湖泊样本中的优势菌属，而变形杆菌属和赖氨酸芽孢杆菌属分别是玄武湖和乌龙潭中第三丰富的菌属。检测了100个分离株中九种抗生素抗性基因（ARGs）的存在情况，且这些分离株中的大多数都携带至少两种具有不同功能的ARGs。革兰氏阴性分离株中ARGs的检测频率高于革兰氏阳性分离株。1 00个分离株中最普遍的整合子是int(II)（n = 28），其次是int(I)（n = 17）和int(III)（n = 17）。我们的结果表明，强降雨事件可能促使ARGs和抗生素抗性细菌从陆地-下水道系统转移至城市湖泊。
