gene resequencing in the Malagasy, a population at the crossroads between Asia and Africa: a pilot study.

malaria is endemic in Madagascar, where populations have genetic inheritance from Southeast Asia and East Africa. Primaquine, a drug of choice for vivax malaria, is metabolized principally via CYP2D6. variation was characterized by locus-specific gene sequencing and was compared with TaqMan™ genotype data. Long-range PCR amplicons were generated from 96 Malagasy samples and subjected to next-generation sequencing. The authors observed high concordance between TaqMan™-based genotype calls and the base calls from sequencing. In addition, there are new variants and haplotypes present in the Malagasy. Sequencing unique admixed populations provides more detailed and accurate insights regarding variability, which may help optimize primaquine treatment across human genetic diversity.