Department of Biomedical Sciences, School of Allied Health Sciences, University of Cape Coast, Cape Coast, Ghana.
Department of Biomedical Sciences, School of Basic and Biomedical Sciences, University of Health and Allied Sciences, Ho, Volta Region, Ghana.
Parasitol Res. 2022 May;121(5):1455-1465. doi: 10.1007/s00436-022-07476-w. Epub 2022 Mar 1.
Blood transfusion practice is an essential medical intervention; however, it poses problems of transmissibility of infectious diseases including malaria. This study was designed to determine the potential of transfusion-transmitted malaria (TTM) by detecting malaria antigens and parasites in recipients of infected donor blood. After successful blood transfusion, remnants of transfused blood were screened for Plasmodium falciparum HRP2 antigen and parasitemia using CareStart malaria RDT and 10% Giemsa stain microscopy respectively according to established protocols. Recipients of microscopy detectable P. falciparum in infected blood who tested negative for malaria by both microscopy and mRDT prior to receiving infected donor blood were followed up weekly for 35 days. Donor P. falciparum antigenemia and parasitemia were 12.1% and 8.4%, respectively, while the prevalence of blood recipient parasitemia was 3.2%. Blood stored for 2-5 days recorded mean parasitemia higher than those stored for a day and after 5 days. Additionally, parasitemia was observed in all follow-up days with marginally high frequencies in days 7, 14, and 35. There was no association between the attributes (storage days, blood group, and parasite count range) of the infected donor blood units and the characteristics of blood recipients with post-transfusion parasitemia. This study provides baseline data on TTM in Ghana. However, further studies should establish the genetic relatedness of the implicated parasites since new infections and/or recrudescence of previous infections could account for this observation.
输血是一种重要的医疗干预措施；然而，它也存在传染病传播的问题，包括疟疾。本研究旨在通过检测受感染供体血液接受者中的疟原虫抗原和寄生虫来确定输血传播疟疾（TTM）的可能性。成功输血后，根据既定方案，分别使用 CareStart 疟疾快速诊断检测（RDT）和 10%吉姆萨染色显微镜检查输血残留血中恶性疟原虫 HRP2 抗原和寄生虫血症。在接受感染供体血液之前，通过显微镜和 mRDT 检测均为阴性的受感染血液中显微镜可检测到恶性疟原虫的接受者，每周随访 35 天。供体恶性疟原虫抗原血症和寄生虫血症分别为 12.1%和 8.4%，而血液接受者寄生虫血症的患病率为 3.2%。储存 2-5 天的血液记录的平均寄生虫血症高于储存 1 天和 5 天后的血液。此外，在所有随访日都观察到寄生虫血症，在第 7、14 和 35 天的频率略高。感染供体血液单位的属性（储存天数、血型和寄生虫计数范围）与输血后寄生虫血症的血液接受者特征之间没有关联。本研究提供了加纳 TTM 的基线数据。然而，应进一步研究确定所涉及寄生虫的遗传相关性，因为新感染和/或以前感染的复发可能导致这种观察结果。
