基因组范围内分析 JAZ 家族基因在无花果（Ficus carica L.）果实发育过程中的表达模式以及对激素处理的响应。

Genome-wide analysis of JAZ family genes expression patterns during fig (Ficus carica L.) fruit development and in response to hormone treatment.

机构信息

College of Horticulture, China Agricultural University, Beijing, 100193, China.

State Key Laboratory of Agrobiotechnology, China Agricultural University, Beijing, 100193, China.

出版信息

BMC Genomics. 2022 Mar 2;23(1):170. doi: 10.1186/s12864-022-08420-z.

BACKGROUND

Jasmonate-ZIM domain (JAZ) repressors negatively regulate signal transduction of jasmonates, which regulate plant development and immunity. However, no comprehensive analysis of the JAZ gene family members has been done in the common fig (Ficus carica L.) during fruit development and hormonal treatment.

RESULTS

In this study, 10 non-redundant fig JAZ family genes (FcJAZs) distributed on 7 chromosomes were identified in the fig genome. Phylogenetic and structural analysis showed that FcJAZ genes can be grouped into 5 classes. All the classes contained relatively complete TIFY and Jas domains. Yeast two hybrid (Y2H) results showed that all FcJAZs proteins may interact with the identified transcription factor, FcMYC2. Tissue-specific expression analysis showed that FcJAZs were highly expressed in the female flowers and roots. Expression patterns of FcJAZs during the fruit development were analyzed by RNA-Seq and qRT-PCR. The findings showed that, most FcJAZs were significantly downregulated from stage 3 to 5 in the female flower, whereas downregulation of these genes was observed in the fruit peel from stage 4 to 5. Weighted-gene co-expression network analysis (WGCNA) showed the expression pattern of FcJAZs was correlated with hormone signal transduction and plant-pathogen interaction. Putative cis-elements analysis of FcJAZs and expression patterns of FcJAZs which respond to hormone treatments revealed that FcJAZs may regulate fig fruit development by modulating the effect of ethylene or gibberellin.

CONCLUSIONS

This study provides a comprehensive analysis of the FcJAZ family members and provides information on FcJAZs contributions and their role in regulating the common fig fruit development.

背景

茉莉酸-ZIM 结构域（JAZ）抑制剂负调控茉莉酸信号转导，茉莉酸调控植物发育和免疫。然而，在果实发育和激素处理过程中，普通榕（Ficus carica L.）中尚未对 JAZ 基因家族成员进行全面分析。

结果

本研究在榕基因组中鉴定了 10 个非冗余的榕 JAZ 家族基因（FcJAZs），分布在 7 条染色体上。系统发育和结构分析表明，FcJAZ 基因可分为 5 类。所有类群均含有较为完整的 TIFY 和 Jas 结构域。酵母双杂交（Y2H）结果表明，所有 FcJAZ 蛋白可能与鉴定的转录因子 FcMYC2 相互作用。组织特异性表达分析表明，FcJAZs 在雌花和根中高表达。通过 RNA-Seq 和 qRT-PCR 分析了 FcJAZs 在果实发育过程中的表达模式。研究结果表明，在雌花中，从第 3 期到第 5 期，大多数 FcJAZs 显著下调，而在果实果皮中，从第 4 期到第 5 期，这些基因下调。加权基因共表达网络分析（WGCNA）表明，FcJAZs 的表达模式与激素信号转导和植物-病原体相互作用有关。对 FcJAZs 的顺式作用元件分析和对激素处理响应的 FcJAZs 的表达模式表明，FcJAZs 可能通过调节乙烯或赤霉素的作用来调节普通榕果实发育。