College of Landscape Architecture, Northeast Forestry University, Harbin, Heilongjiang, China.
College of Horticulture, Jilin Agricultural University, Jilin, China.
PeerJ. 2022 Oct 27;10:e14241. doi: 10.7717/peerj.14241. eCollection 2022.
promoter-binding-protein (SBP)-box family proteins are a class of plant-specific transcription factors, and widely regulate the development of floral and leaf morphology in plant growth and involve in environment and hormone signal response. In this study, we isolated and identified 21 non-redundant genes in with bioinformatics analysis. Sequence alignments of 21 CnSBP proteins discovered a highly conserved SBP domain including two zinc finger-like structures and a nuclear localization signal region. According to the amino acid sequence alignments, 67 genes from , rice, and were clustered into eight groups, and the motif and gene structure analysis also sustained this classification. The gene evolution analysis indicated the genes experienced a duplication event about 10 million years ago (Mya), and the and genes occurred a divergence at 24 Mya. Transcriptome data provided valuable information for tissue-specific expression profiles of the , which highly expressed in floral tissues and differentially expressed in leaf, root and stem organs. Quantitative Real-time Polymerase Chain Reaction data showed expression patterns of the under exogenous hormone and abiotic stress treatments, separately abscisic acid, salicylic acid, gibberellin A3, methyl jasmonate and ethylene spraying as well as salt and drought stresses, indicating that the candidate genes showed differentiated spatiotemporal expression patterns in response to hormone and abiotic stresses. Our study provides a systematic genome-wide analysis of the gene family in . In general, it provides a fundamental theoretical basis that genes may regulate the resistance of stress physiology in chrysanthemum via exogenous hormone pathways.
启动子结合蛋白（SBP）-盒家族蛋白是一类植物特异性转录因子，广泛调节植物生长过程中花和叶形态的发育，并参与环境和激素信号响应。在这项研究中，我们通过生物信息学分析从 中分离和鉴定了 21 个非冗余基因。对 21 个 CnSBP 蛋白的序列比对发现了一个高度保守的 SBP 结构域，包括两个锌指样结构和一个核定位信号区。根据氨基酸序列比对，来自 、水稻、 和 的 67 个基因被聚类为 8 个组， motif 和基因结构分析也支持了这种分类。基因进化分析表明， 基因在大约 1000 万年前（Mya）经历了一次复制事件，而 和 基因在 2400 万年前发生了分化。转录组数据为 基因的组织特异性表达谱提供了有价值的信息，该基因在花组织中高度表达，并在叶、根和茎器官中表现出差异表达。定量实时聚合酶链反应数据显示， 基因在外源激素和非生物胁迫处理下的表达模式，分别为脱落酸、水杨酸、赤霉素 A3、茉莉酸甲酯和乙烯喷雾以及盐和干旱胁迫，表明候选 基因在外源激素和非生物胁迫下表现出不同的时空表达模式。我们的研究提供了菊花 基因家族的系统全基因组分析。总的来说，它为 基因可能通过外源激素途径调节菊花胁迫生理抗性提供了一个基本的理论基础。
