Hunan Plant Protection Institute, Hunan Academy of Agricultural Science, Changsha, 410125, Hunan, People's Republic of China.
Crop Research Institute, Hunan Academy of Agricultural Science, Changsha, 410125, Hunan, People's Republic of China.
Sci Rep. 2022 Mar 2;12(1):3470. doi: 10.1038/s41598-022-07119-1.
The dissipation and residue levels of thiacloprid, spirotetramat and its four metabolites residues in cowpeas were investigated under field conditions. The QuEChERS technique with high-performance liquid chromatography tandem mass spectrometry (HPLC-MS/MS) was used to detect thiacloprid, spirotetramat and its four metabolites residues content in cowpeas. The recoveries were 81.3-95.1% at a spike level of 0.005-0.5 mg/kg, the relative standard deviations (RSDs) were 2.1-9.5%. The dissipation kinetics data showed that thiacloprid and spirotetramat in cowpeas were degraded with the half-lives of 1.14-1.54 days and 1.25-2.79 days. The terminal residues of thiacloprid and spirotetramat were 0.0255-0.4570 mg kg and 0.0314-0.3070 mg kg after application 2 times with a pre-harvest interval (PHI) of 3 days under the designed dosages. The chronic and acute dietary exposure assessment risk quotient (RQ) values of thiacloprid in cowpeas for different consumers were 2.44-4.41% and 8.72-15.78%, respectively, and those of spirotetramat were 1.03-1.87% and 0.18-0.32%, respectively, all of the RQ values were lower than 100%. The dietary risk of thiacloprid through cowpeas to consumers was higher than spirotetramat. The results from this study are important reference for Chinese governments to develop criteria for the safe and rational use of thiacloprid and spirotetramat, setting maximum residue levels (MRLs), monitoring the quality safety of agricultural products and protecting consumer health.
在田间条件下研究了噻虫啉、螺虫乙酯及其四种代谢物在豇豆中的消解和残留水平。采用 QuEChERS 技术结合高效液相色谱-串联质谱法（HPLC-MS/MS）检测豇豆中噻虫啉、螺虫乙酯及其四种代谢物的残留量。在 0.005-0.5mg/kg 的添加水平下，回收率为 81.3-95.1%，相对标准偏差（RSD）为 2.1-9.5%。消解动力学数据表明，噻虫啉和螺虫乙酯在豇豆中的降解半衰期分别为 1.14-1.54 天和 1.25-2.79 天。在设计剂量下，施药 2 次，间隔 3 天，末次残留量分别为 0.0255-0.4570mg/kg 和 0.0314-0.3070mg/kg。不同消费者食用豇豆中噻虫啉的慢性和急性膳食暴露评估风险商（RQ）值分别为 2.44-4.41%和 8.72-15.78%，螺虫乙酯分别为 1.03-1.87%和 0.18-0.32%，RQ 值均低于 100%。消费者通过食用豇豆摄入噻虫啉的膳食风险高于螺虫乙酯。本研究结果为中国政府制定噻虫啉和螺虫乙酯安全合理使用标准、设定最大残留限量（MRLs）、监测农产品质量安全和保护消费者健康提供了重要参考。
