Department of Biological Sciences, University of Limerick, Limerick, Ireland.
Health Research Institute, University of Limerick, Limerick, Ireland.
Methods Mol Biol. 2022;2419:333-347. doi: 10.1007/978-1-0716-1924-7_21.
Platelet activation and aggregation is implicated in all stages of inflammation-related atherosclerosis from the initial steps of endothelial dysfunction and plaque formation, to plaque rupture and atherothrombotic events, such as acute coronary syndrome, myocardial infarction, and ischemic incidences. Platelet aggregometry assays are the mainstream for evaluating and monitoring platelet reactivity in such conditions and for the investigation of prophylactic and therapeutic approaches. The most established methodology is light transmittance aggregometry (LTA). Here we describe the appropriate preparation of platelet suspensions from human blood and the methodology of LTA-based assays that is used for basic and clinical research for monitoring and evaluating the activities of several thrombotic mediators, as well as determining the dose efficacy and safety of several pharmaceutical and nutraceutical compounds intended for therapeutic and prophylactic interventions for atherosclerosis.
血小板的激活和聚集与炎症相关动脉粥样硬化的所有阶段都有关，从内皮功能障碍和斑块形成的初始步骤，到斑块破裂和动脉粥样硬化血栓形成事件，如急性冠状动脉综合征、心肌梗死和缺血性事件。血小板聚集测定法是评估和监测此类情况下血小板反应性以及研究预防和治疗方法的主流方法。最成熟的方法是光透射聚集测定法（LTA）。在这里，我们描述了从人血中适当制备血小板混悬液的方法，以及用于基础和临床研究的基于 LTA 的测定方法，用于监测和评估几种血栓形成介质的活性，以及确定几种用于动脉粥样硬化治疗和预防干预的药物和营养化合物的剂量效果和安全性。
