动脉粥样硬化斑块易损性研究。

Investigation of Atherosclerotic Plaque Vulnerability.

机构信息

Department of Translational Health Sciences, Bristol Medical School, University of Bristol, Bristol, UK.

出版信息

Methods Mol Biol. 2022;2419:521-535. doi: 10.1007/978-1-0716-1924-7_32.

DOI:10.1007/978-1-0716-1924-7_32

Abstract

Histochemical and immunohistochemical approaches permit the detection and evaluation of proteins and cell types within murine brachiocephalic artery atherosclerotic plaques, that can be subsequently analyzed to provide inferences on atherosclerotic plaque vulnerability. Here we describe the specific histochemical techniques deployed to examine the expression of elastin, fibrillar collagens, and neutral lipids, alongside immunohistochemistry protocols for the identification of macrophages (CD68) and vascular smooth muscle cells (α-smooth muscle actin). We will also describe how analyses derived from these methods can be combined to determine evidence of previous plaque rupture and susceptibility to rupture.

摘要

组织化学和免疫组织化学方法可用于检测和评估小鼠头臂动脉粥样硬化斑块中的蛋白质和细胞类型，随后对这些斑块进行分析，以提供关于易损性的推断。在这里，我们描述了用于检查弹性蛋白、纤维胶原和中性脂质表达的具体组织化学技术，以及用于鉴定巨噬细胞（CD68）和血管平滑肌细胞（α-平滑肌肌动蛋白）的免疫组织化学方案。我们还将描述如何结合这些方法的分析来确定先前斑块破裂的证据和易破裂的倾向。