Centro Nacional de Investigaciones Cardiovasculares (CNIC), Madrid, Spain.
Centro de Investigación Biomédica en Red de Enfermedades Cardiovasculares (CIBERCV), Madrid, Spain.
Methods Mol Biol. 2022;2419:611-627. doi: 10.1007/978-1-0716-1924-7_38.
Aging is associated with alterations in the arterial wall that promote vascular disease development and its clinical manifestations, including myocardial infarction, stroke, and arterial dissection. The arterial wall is comprised of three layers, intima, media and adventitia, each with distinct cellular composition and function, which can therefore contribute differently to vascular disease initiation and progression. Hence, studying transcriptomic alterations, either in the entire arterial wall or separately in the three arterial layers, can aid in disentangling the etiopathology of vascular disease and thus pave the way for innovative treatments. This chapter describes protocols for total RNA extraction from complete mouse aorta and separately from intima, media, and adventitia layers for subsequent transcriptomic analysis.
衰老是动脉壁改变的相关因素，促进血管疾病的发展及其临床表现，包括心肌梗死、中风和动脉夹层。动脉壁由内膜、中膜和外膜三层组成，每层都有独特的细胞组成和功能，因此对血管疾病的起始和进展有不同的影响。因此，研究整个动脉壁或三个动脉层中单独的转录组变化，可以帮助阐明血管疾病的病因，并为创新治疗铺平道路。本章描述了从完整的小鼠主动脉和内膜、中膜和外膜层分别提取总 RNA 的方案，用于随后的转录组分析。
