Institute of Biosciences, Universidade Estadual Paulista Júlio de Mesquita Filho, Botucatu, São Paulo, Brazil.
Human Performance Laboratory, Universidade Metodista de Piracicaba, Piracicaba, São Paulo, Brazil.
J Hum Nutr Diet. 2022 Aug;35(4):731-738. doi: 10.1111/jhn.13000. Epub 2022 Mar 29.
Although effective, the impact of bariatric surgery on weight loss is variable, and little is known about the influence of single-nucleotide polymorphisms (SNPs). The present study investigated the association of eleven SNPs related to obesity with weight loss 1 year after Roux-Y gastric bypass (RYGB) surgery in female patients.
This prospective study included 351 women with obesity. The genotypes for eleven SNPs (GHRL-rs26802; GHSR-rs572169; LEP-rs7799039; LEPR-rs1137101; 5HT2C-rs3813929; UCP2-rs659366; UCP3-rs1800849; SH2B1-rs7498665; TAS1R2-rs35874116; TAS1R2-rs9701796; FTO-rs9939609) were determined using a real-time polymerase chain reaction and TaqMan assays. Anthropometric measurements were performed before and 1 year after RYGB surgery. To evaluate the factors that influenced the proportion of weight loss 1 year after surgery, beta regression analysis was used. The models were estimated using the GLIMMIX procedure in SAS statistical software. p < 0.05 was considered statistically significant.
The mean ± SD percentage of total body weight loss in 1 year was 64.4 ± 5.8% and the median was 65.0%. When assessing the proportion of weight loss in 1 year after surgery, using univariate analysis (beta regression), no SNPs influenced weight loss. Furthermore, in the multiple analysis, with stepwise process of variable selection, no variable was significant to compose the multiple model.
The 11 SNPs investigated did not influence weight loss 1 year after RYGB surgery in female patients. This result indicates that individual behaviours and other factors might better contribute to the magnitude of loss weight loss in a short period after bariatric surgery.
尽管减重手术效果显著，但术后体重减轻的效果因人而异，目前人们对单核苷酸多态性（SNP）的影响知之甚少。本研究旨在探讨女性患者行 Roux-Y 胃旁路术（RYGB）后 1 年，11 个与肥胖相关的 SNP 与减重的相关性。
本前瞻性研究纳入了 351 例肥胖女性患者。采用实时聚合酶链反应和 TaqMan 检测技术，对 11 个 SNP（GHRL-rs26802；GHSR-rs572169；LEP-rs7799039；LEPR-rs1137101；5HT2C-rs3813929；UCP2-rs659366；UCP3-rs1800849；SH2B1-rs7498665；TAS1R2-rs35874116；TAS1R2-rs9701796；FTO-rs9939609）的基因型进行了测定。在 RYGB 术前和术后 1 年，进行了人体测量。采用贝叶斯回归分析评估影响术后 1 年体重减轻比例的因素。模型采用 SAS 统计软件的 GLIMMIX 程序进行估计。p<0.05 为差异有统计学意义。
术后 1 年的体重总减轻率的平均值±标准差为 64.4±5.8%，中位数为 65.0%。单因素分析（贝叶斯回归）发现，11 个 SNP 均不影响术后 1 年的体重减轻率。进一步采用多因素分析，采用逐步变量选择法，未发现有变量可纳入多因素模型。
在女性患者中，11 个 SNP 均不影响 RYGB 术后 1 年的体重减轻率。这一结果表明，在减重手术后的短时间内，个体行为和其他因素可能对体重减轻的幅度有更好的影响。
