Tianjin Key Laboratory of Risk Assessment and Control Technology for Environment and Food Safety, Tianjin Institute of Environmental and Operational Medicine, Tianjin, 300050, PR China.
Tianjin Key Laboratory of Risk Assessment and Control Technology for Environment and Food Safety, Tianjin Institute of Environmental and Operational Medicine, Tianjin, 300050, PR China.
Talanta. 2022 Jun 1;243:123338. doi: 10.1016/j.talanta.2022.123338. Epub 2022 Feb 26.
Swift and effective diagnosis of acute myocardial infarction (AMI) is critical to patient survival due to its serious life-threatening effects and increasing incidence. Creatine kinase MB (CK-MB) is one of the markers of AMI. In this work, we enabled a portable visual quantitative assay of CK-MB by incorporating target-responsive DNA hydrogel with a microfluidic chip. The CK-MB aptamer and the complementary short DNA strand were grafted onto the polyacrylamide strand separately and formed the hydrogel by base-paired linkage. Upon introduction of CK-MB, the aptamer bound to CK-MB. This led to hydrogel dissociation and subsequent release of pre-trapped gold nanoparticles (AuNPs), which is proportional to the concentration of CK-MB. To achieve portable on-site detection, we further combined the hydrogel with a microfluidic chip and utilized the color change caused by the released AuNPs to take picture and analyze the average gray value. Then, as low as 0.027 nM CK-MB could be detected by cell phone. With good portability, visualization, and simple sample handling, this method has great potential for quantitative point-of-care testing (POCT) of targets in resource-constrained settings.
快速有效地诊断急性心肌梗死（AMI）至关重要，因为它会产生严重的危及生命的影响，且发病率不断上升。肌酸激酶同工酶 MB（CK-MB）是 AMI 的标志物之一。在这项工作中，我们通过将靶标响应性 DNA 水凝胶与微流控芯片结合，实现了对 CK-MB 的便携式可视化定量分析。CK-MB 适体和互补的短 DNA 链分别接枝到聚丙烯酰胺链上，并通过碱基配对连接形成水凝胶。当引入 CK-MB 时，适体与 CK-MB 结合。这导致水凝胶解离，随后预先捕获的金纳米粒子（AuNP）释放，这与 CK-MB 的浓度成正比。为了实现便携式现场检测，我们进一步将水凝胶与微流控芯片结合，并利用释放的 AuNPs 引起的颜色变化拍照并分析平均灰度值。然后，通过手机可以检测到低至 0.027 nM 的 CK-MB。该方法具有良好的便携性、可视化和简单的样本处理，有望在资源有限的环境中实现针对目标的定量即时检测（POCT）。
