Bondarenko V M, Zhalko-Titarenko V P, Grigor'ev A V, Kupchinskiĭ L G, Koval'chuk V K
Zh Mikrobiol Epidemiol Immunobiol. 1986 May(5):29-32.
In experiments on human fetal intestinal explants infected with shigellae the specific multiplication rate of these infective agents, found to be 0.026, and the maximum level of their accumulation in erythrocytes, reaching 22-36 microbes per cell, have been determined. These phenomena can be observed after at least 3-hour incubation and end in the release of the infective agents from the affected area with shedding epithelial elements (villi). Shigellae, aggregated in the shed villi easily adhering to the unaffected mucosa, ensure the intensive invasion of the epithelium, which leads to the continuation of the process. The regularity thus revealed indicates that the population cycle of the development of shigellae is limited by short intervals of 3-4 hours. During these intervals the repeated invasion and the release of shigellae, together with the shed epithelium, into the chyme-containing intestinal cavity occur. The conditions for the multiplication of shigellae and their specific multiplication rate in chyme are minimal (0.016).
在对感染志贺氏菌的人胎儿肠道外植体进行的实验中，已确定这些感染因子的特定增殖率为0.026，以及它们在红细胞中的最大积累水平，达到每个细胞22 - 36个微生物。这些现象至少在3小时孵育后即可观察到，并随着受影响区域上皮成分（绒毛）的脱落，感染因子从该区域释放而结束。聚集在脱落绒毛中的志贺氏菌很容易附着在未受影响的黏膜上，确保上皮细胞的强烈侵袭，这导致了该过程的持续。由此揭示的规律表明，志贺氏菌发育的种群周期受限于3 - 4小时的短间隔。在这些间隔期间，志贺氏菌会反复侵袭并与脱落的上皮细胞一起释放到含有食糜的肠腔中。志贺氏菌在食糜中的增殖条件及其特定增殖率最低（0.016）。
