School of Agriculture, Tokai University, Kumamoto, Japan;
Swine and Poultry Department, Swine and Poultry Research Center, Shizuoka Prefectural Research Institute of Animal Industry, Shizuoka, Japan.
In Vivo. 2022 Mar-Apr;36(2):731-737. doi: 10.21873/invivo.12759.
BACKGROUND/AIM: The microminipig is a relatively new type of mini pig; microminipigs weigh about 10 kg at 6 months of age and are expected to be of use in drug discovery research and safety tests. Herein, we analyzed the characteristics of ejaculated sperm from microminipigs.
Sperm parameters such as microstructure and sensitivity to cold shock were investigated using optical or scanning electron microscopy.
Ejaculate volumes and total numbers of sperm were lower than in standard pig strains, but were proportional to body weight. Ejaculation time, pH of the ejaculate, sperm motility and morphology, and sensitivity to cold shock were similar to those of standard pig strains.
Herein, we provide the first characterization of the ejaculates of microminipigs and demonstrate that this type of pig will be useful not only in medical research, but also in investigations into sperm preservation in different pig breeds.
背景/目的：微型迷你猪是一种较新的小型猪品种；微型迷你猪在 6 月龄时体重约为 10 公斤，有望在药物发现研究和安全性测试中发挥作用。在此，我们分析了微型迷你猪射出精液的特征。
使用光学或扫描电子显微镜观察精液的微观结构和对冷休克的敏感性等精子参数。
射精量和精子总数低于标准猪种，但与体重成比例。射精时间、精液 pH 值、精子活力和形态以及对冷休克的敏感性与标准猪种相似。
在此，我们首次对微型迷你猪的精液进行了特征描述，并证明这种猪不仅在医学研究中有用，而且在不同猪种的精子保存研究中也有用。
