一种在流动系统中使扁平细胞定向的方法。
A means for orienting flat cells in flow systems.
作者信息
Stovel R T, Sweet R G, Herzenberg L A
出版信息
Biophys J. 1978 Jul;23(1):1-5. doi: 10.1016/S0006-3495(78)85427-7.
Flattened cells, such as red blood cells, epithelial cells, and sperm of many species, cause problems for fluorescence-activated cell analysis and sorting machines because the flow systems of such devices are unable to control the orientation of these cells as they flow past the detectors. For this reason, the fluorescence or scattered light measurements for identical cells may vary greatly. A flow geometry is here described that orients flat cells in a coaxial flow system so that each cell presents the same aspect to the observation device. A wedge-shaped exit on the sample injection tube in a coaxial flow system is sufficient to produce the desired orientation effect when used with low sample flow rates. Data is presented showing the effect of orientation of fixed chicken erythrocytes on histograms of small forward-angle light-scattering measurements.
扁平细胞，如许多物种的红细胞、上皮细胞和精子，给荧光激活细胞分析和分选机器带来了问题，因为这类设备的流动系统在这些细胞流经探测器时无法控制其方向。因此，相同细胞的荧光或散射光测量结果可能会有很大差异。本文描述了一种流动几何学方法，可使扁平细胞在同轴流动系统中定向，以便每个细胞都以相同的面朝向观测设备。当与低样品流速一起使用时，同轴流动系统中样品注入管上的楔形出口足以产生所需的定向效果。文中给出的数据显示了固定鸡红细胞的定向对小前向角光散射测量直方图的影响。
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