用于流式细胞术的精子头部流体动力学定向
Hydrodynamic orientation of sperm heads for flow cytometry.
作者信息
Dean P N, Pinkel D, Mendelsohn M L
出版信息
Biophys J. 1978 Jul;23(1):7-13. doi: 10.1016/S0006-3495(78)85428-9.
Abstract
Two specially shaped sample injection tubes orient flat-shaped mature sperm heads in a flow cytometer. Orientation allows control of a photometric difficulty experienced with some flow cytometers in the measurement of fluorescent and scattered light from sperm and other flat cells. Both relative DNA-stain content and a measure of cell flatness can now be determined simultaneously for each cell at the high rates possible with flow instruments.
摘要
两个特殊形状的样品注射管可在流式细胞仪中使扁平状的成熟精子头部定向。这种定向能够控制一些流式细胞仪在测量精子和其他扁平细胞的荧光和散射光时所遇到的光度测量难题。现在，利用流式仪器的高速率，能够同时为每个细胞确定相对DNA染色含量和细胞扁平度的测量值。
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